Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26390 and BPHYT_RS26395 are separated by 373 nucleotides BPHYT_RS26395 and BPHYT_RS26400 overlap by 4 nucleotides BPHYT_RS26400 and BPHYT_RS26405 are separated by 318 nucleotides
BPHYT_RS26390: BPHYT_RS26390 - antibiotic resistance protein, at 1,493,940 to 1,494,617
_RS26390
BPHYT_RS26395: BPHYT_RS26395 - membrane protein, at 1,494,991 to 1,495,206
_RS26395
BPHYT_RS26400: BPHYT_RS26400 - sodium:solute symporter, at 1,495,203 to 1,496,675
_RS26400
BPHYT_RS26405: BPHYT_RS26405 - glycogen synthase, at 1,496,994 to 1,498,583
_RS26405
Position (kb) 1495
1496
1497 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1494.617 kb on + strand at 1494.777 kb on + strand at 1494.857 kb on - strand at 1494.928 kb on + strand at 1494.964 kb on + strand at 1494.964 kb on + strand at 1495.450 kb on - strand, within BPHYT_RS26400 at 1495.707 kb on + strand, within BPHYT_RS26400 at 1495.815 kb on + strand, within BPHYT_RS26400 at 1496.247 kb on + strand, within BPHYT_RS26400 at 1496.573 kb on + strand at 1496.696 kb on + strand at 1496.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,494,617 + +0.6 1,494,777 + +0.9 1,494,857 - +0.0 1,494,928 + +0.0 1,494,964 + +2.3 1,494,964 + +0.6 1,495,450 - BPHYT_RS26400 0.17 -1.3 1,495,707 + BPHYT_RS26400 0.34 +0.4 1,495,815 + BPHYT_RS26400 0.42 -1.4 1,496,247 + BPHYT_RS26400 0.71 -2.7 1,496,573 + +0.3 1,496,696 + -0.3 1,496,828 - +0.5
Or see this region's nucleotide sequence