Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25015

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25010 and BPHYT_RS25015 are separated by 239 nucleotidesBPHYT_RS25015 and BPHYT_RS25020 are separated by 138 nucleotidesBPHYT_RS25020 and BPHYT_RS25025 are separated by 305 nucleotides BPHYT_RS25010: BPHYT_RS25010 - N5,N10-methylene tetrahydromethanopterin reductase, at 1,180,617 to 1,182,008 _RS25010 BPHYT_RS25015: BPHYT_RS25015 - XRE family transcriptional regulator, at 1,182,248 to 1,182,817 _RS25015 BPHYT_RS25020: BPHYT_RS25020 - phospholipid-binding protein, at 1,182,956 to 1,183,315 _RS25020 BPHYT_RS25025: BPHYT_RS25025 - acyl-CoA dehydrogenase, at 1,183,621 to 1,185,426 _RS25025 Position (kb) 1182 1183Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1181.336 kb on - strand, within BPHYT_RS25010at 1181.441 kb on - strand, within BPHYT_RS25010at 1181.503 kb on + strand, within BPHYT_RS25010at 1181.504 kb on - strand, within BPHYT_RS25010at 1181.519 kb on - strand, within BPHYT_RS25010at 1181.672 kb on - strand, within BPHYT_RS25010at 1181.672 kb on - strand, within BPHYT_RS25010at 1181.897 kb on - strandat 1181.901 kb on + strandat 1181.909 kb on + strandat 1181.910 kb on - strandat 1182.097 kb on - strandat 1182.201 kb on - strandat 1182.513 kb on + strand, within BPHYT_RS25015at 1182.513 kb on + strand, within BPHYT_RS25015at 1182.707 kb on + strand, within BPHYT_RS25015at 1182.815 kb on + strandat 1182.815 kb on + strandat 1182.829 kb on + strandat 1183.248 kb on + strand, within BPHYT_RS25020at 1183.414 kb on - strandat 1183.448 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,181,336 - BPHYT_RS25010 0.52 -0.9
1,181,441 - BPHYT_RS25010 0.59 -0.6
1,181,503 + BPHYT_RS25010 0.64 -0.3
1,181,504 - BPHYT_RS25010 0.64 +0.5
1,181,519 - BPHYT_RS25010 0.65 -0.3
1,181,672 - BPHYT_RS25010 0.76 -1.1
1,181,672 - BPHYT_RS25010 0.76 -1.4
1,181,897 - -0.8
1,181,901 + +0.4
1,181,909 + +0.2
1,181,910 - -0.6
1,182,097 - +0.5
1,182,201 - +0.3
1,182,513 + BPHYT_RS25015 0.46 -4.2
1,182,513 + BPHYT_RS25015 0.46 +1.0
1,182,707 + BPHYT_RS25015 0.81 -0.2
1,182,815 + -2.2
1,182,815 + -0.0
1,182,829 + -1.8
1,183,248 + BPHYT_RS25020 0.81 -0.7
1,183,414 - -0.2
1,183,448 - +0.2

Or see this region's nucleotide sequence