Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23500

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23495 and BPHYT_RS23500 are separated by 134 nucleotidesBPHYT_RS23500 and BPHYT_RS23505 are separated by 214 nucleotidesBPHYT_RS23505 and BPHYT_RS23510 are separated by 164 nucleotides BPHYT_RS23495: BPHYT_RS23495 - thioredoxin reductase, at 836,967 to 838,700 _RS23495 BPHYT_RS23500: BPHYT_RS23500 - hypothetical protein, at 838,835 to 840,013 _RS23500 BPHYT_RS23505: BPHYT_RS23505 - hypothetical protein, at 840,228 to 840,611 _RS23505 BPHYT_RS23510: BPHYT_RS23510 - AMP-dependent synthetase, at 840,776 to 842,320 _RS23510 Position (kb) 838 839 840 841Strain fitness (log2 ratio) -2 -1 0 1at 837.994 kb on - strand, within BPHYT_RS23495at 838.002 kb on - strand, within BPHYT_RS23495at 838.132 kb on - strand, within BPHYT_RS23495at 839.286 kb on + strand, within BPHYT_RS23500at 840.016 kb on - strandat 840.116 kb on + strandat 840.116 kb on + strandat 840.151 kb on - strandat 840.616 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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837,994 - BPHYT_RS23495 0.59 +0.1
838,002 - BPHYT_RS23495 0.60 +1.0
838,132 - BPHYT_RS23495 0.67 -1.9
839,286 + BPHYT_RS23500 0.38 +0.5
840,016 - -0.9
840,116 + -1.6
840,116 + +1.3
840,151 - -1.3
840,616 + +1.3

Or see this region's nucleotide sequence