Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23085 and BPHYT_RS23090 are separated by 33 nucleotides BPHYT_RS23090 and BPHYT_RS23095 are separated by 37 nucleotides BPHYT_RS23095 and BPHYT_RS23100 are separated by 26 nucleotides
BPHYT_RS23085: BPHYT_RS23085 - MFS transporter, at 743,089 to 744,501
_RS23085
BPHYT_RS23090: BPHYT_RS23090 - histidine kinase, at 744,535 to 745,089
_RS23090
BPHYT_RS23095: BPHYT_RS23095 - MFS transporter, at 745,127 to 746,359
_RS23095
BPHYT_RS23100: BPHYT_RS23100 - sugar ABC transporter substrate-binding protein, at 746,386 to 747,333
_RS23100
Position (kb) 745
746
747 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 744.202 kb on + strand, within BPHYT_RS23085 at 744.203 kb on - strand, within BPHYT_RS23085 at 744.209 kb on - strand, within BPHYT_RS23085 at 744.915 kb on - strand, within BPHYT_RS23090 at 745.221 kb on - strand at 745.221 kb on - strand at 745.508 kb on + strand, within BPHYT_RS23095 at 745.634 kb on + strand, within BPHYT_RS23095 at 745.868 kb on + strand, within BPHYT_RS23095 at 745.929 kb on - strand, within BPHYT_RS23095 at 746.186 kb on - strand, within BPHYT_RS23095 at 746.330 kb on + strand at 746.387 kb on + strand at 746.388 kb on - strand at 746.388 kb on - strand at 746.512 kb on + strand, within BPHYT_RS23100 at 746.513 kb on - strand, within BPHYT_RS23100
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 744,202 + BPHYT_RS23085 0.79 -0.9 744,203 - BPHYT_RS23085 0.79 +1.3 744,209 - BPHYT_RS23085 0.79 -0.8 744,915 - BPHYT_RS23090 0.68 -0.9 745,221 - -0.8 745,221 - -0.3 745,508 + BPHYT_RS23095 0.31 +3.0 745,634 + BPHYT_RS23095 0.41 -0.7 745,868 + BPHYT_RS23095 0.60 +0.1 745,929 - BPHYT_RS23095 0.65 +0.0 746,186 - BPHYT_RS23095 0.86 -0.4 746,330 + -0.4 746,387 + -3.3 746,388 - -1.1 746,388 - +0.4 746,512 + BPHYT_RS23100 0.13 -2.9 746,513 - BPHYT_RS23100 0.13 -1.1
Or see this region's nucleotide sequence