Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22840 and BPHYT_RS22845 are separated by 145 nucleotides BPHYT_RS22845 and BPHYT_RS22850 are separated by 213 nucleotides BPHYT_RS22850 and BPHYT_RS22855 are separated by 210 nucleotides BPHYT_RS22855 and BPHYT_RS22860 are separated by 126 nucleotides
BPHYT_RS22840: BPHYT_RS22840 - motility protein B, at 684,247 to 684,915
_RS22840
BPHYT_RS22845: BPHYT_RS22845 - hypothetical protein, at 685,061 to 685,309
_RS22845
BPHYT_RS22850: BPHYT_RS22850 - thioredoxin, at 685,523 to 686,089
_RS22850
BPHYT_RS22855: BPHYT_RS22855 - aldehyde-activating protein, at 686,300 to 686,671
_RS22855
BPHYT_RS22860: BPHYT_RS22860 - chemotaxis protein CheY, at 686,798 to 687,538
_RS22860
Position (kb) 685
686
687 Strain fitness (log2 ratio) -1
0
1
2
3 at 684.731 kb on - strand, within BPHYT_RS22840 at 684.955 kb on + strand at 684.955 kb on + strand at 685.597 kb on - strand, within BPHYT_RS22850 at 685.653 kb on + strand, within BPHYT_RS22850 at 686.152 kb on - strand at 686.152 kb on - strand at 686.557 kb on + strand, within BPHYT_RS22855 at 686.612 kb on + strand, within BPHYT_RS22855 at 686.613 kb on - strand, within BPHYT_RS22855 at 686.615 kb on - strand, within BPHYT_RS22855 at 686.817 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 684,731 - BPHYT_RS22840 0.72 -1.1 684,955 + -0.0 684,955 + -1.6 685,597 - BPHYT_RS22850 0.13 -0.6 685,653 + BPHYT_RS22850 0.23 -0.2 686,152 - +0.1 686,152 - +0.6 686,557 + BPHYT_RS22855 0.69 -0.3 686,612 + BPHYT_RS22855 0.84 +0.6 686,613 - BPHYT_RS22855 0.84 -0.4 686,615 - BPHYT_RS22855 0.85 +0.6 686,817 + +2.9
Or see this region's nucleotide sequence