Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21645 and BPHYT_RS21650 are separated by 31 nucleotides BPHYT_RS21650 and BPHYT_RS21655 overlap by 4 nucleotides BPHYT_RS21655 and BPHYT_RS21660 are separated by 33 nucleotides
BPHYT_RS21645: BPHYT_RS21645 - LysR family transcriptional regulator, at 410,818 to 411,645
_RS21645
BPHYT_RS21650: BPHYT_RS21650 - cobalamin synthesis protein P47K, at 411,677 to 412,609
_RS21650
BPHYT_RS21655: BPHYT_RS21655 - amidohydrolase, at 412,606 to 414,264
_RS21655
BPHYT_RS21660: BPHYT_RS21660 - sodium:solute symporter, at 414,298 to 415,683
_RS21660
Position (kb) 412
413
414
415 Strain fitness (log2 ratio) -2
-1
0
1
2 at 411.927 kb on + strand, within BPHYT_RS21650 at 411.927 kb on + strand, within BPHYT_RS21650 at 412.540 kb on + strand at 412.748 kb on - strand at 412.771 kb on - strand at 413.001 kb on + strand, within BPHYT_RS21655 at 413.002 kb on - strand, within BPHYT_RS21655 at 413.022 kb on - strand, within BPHYT_RS21655 at 413.115 kb on + strand, within BPHYT_RS21655 at 413.999 kb on + strand, within BPHYT_RS21655 at 414.000 kb on - strand, within BPHYT_RS21655 at 414.081 kb on + strand, within BPHYT_RS21655 at 414.082 kb on - strand, within BPHYT_RS21655 at 414.185 kb on + strand at 414.238 kb on - strand at 414.534 kb on - strand, within BPHYT_RS21660 at 414.536 kb on + strand, within BPHYT_RS21660 at 414.867 kb on - strand, within BPHYT_RS21660 at 415.143 kb on + strand, within BPHYT_RS21660 at 415.179 kb on - strand, within BPHYT_RS21660
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 411,927 + BPHYT_RS21650 0.27 +0.8 411,927 + BPHYT_RS21650 0.27 -0.7 412,540 + +0.6 412,748 - +0.1 412,771 - -0.6 413,001 + BPHYT_RS21655 0.24 +0.5 413,002 - BPHYT_RS21655 0.24 -0.4 413,022 - BPHYT_RS21655 0.25 -1.5 413,115 + BPHYT_RS21655 0.31 -1.6 413,999 + BPHYT_RS21655 0.84 -0.8 414,000 - BPHYT_RS21655 0.84 -0.2 414,081 + BPHYT_RS21655 0.89 -1.8 414,082 - BPHYT_RS21655 0.89 -0.1 414,185 + +2.7 414,238 - -0.4 414,534 - BPHYT_RS21660 0.17 +0.4 414,536 + BPHYT_RS21660 0.17 +0.4 414,867 - BPHYT_RS21660 0.41 +0.2 415,143 + BPHYT_RS21660 0.61 -0.6 415,179 - BPHYT_RS21660 0.64 -0.2
Or see this region's nucleotide sequence