Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21570 and BPHYT_RS21575 are separated by 567 nucleotides BPHYT_RS21575 and BPHYT_RS21580 overlap by 11 nucleotides BPHYT_RS21580 and BPHYT_RS21585 are separated by 8 nucleotides BPHYT_RS21585 and BPHYT_RS21590 are separated by 40 nucleotides
BPHYT_RS21570: BPHYT_RS21570 - oxidoreductase, at 398,332 to 399,021
_RS21570
BPHYT_RS21575: BPHYT_RS21575 - hypothetical protein, at 399,589 to 399,789
_RS21575
BPHYT_RS21580: BPHYT_RS21580 - hypothetical protein, at 399,779 to 399,967
_RS21580
BPHYT_RS21585: BPHYT_RS21585 - short-chain dehydrogenase, at 399,976 to 400,737
_RS21585
BPHYT_RS21590: BPHYT_RS21590 - NmrA family transcriptional regulator, at 400,778 to 401,656
_RS21590
Position (kb)
399
400
401 Strain fitness (log2 ratio)
-1
0
1
2 at 399.118 kb on + strand at 399.119 kb on - strand at 399.258 kb on - strand at 399.468 kb on + strand at 399.627 kb on + strand, within BPHYT_RS21575 at 399.827 kb on + strand, within BPHYT_RS21580 at 400.167 kb on + strand, within BPHYT_RS21585 at 400.314 kb on - strand, within BPHYT_RS21585 at 400.329 kb on + strand, within BPHYT_RS21585 at 400.421 kb on - strand, within BPHYT_RS21585 at 400.710 kb on + strand at 401.077 kb on + strand, within BPHYT_RS21590
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 399,118 + +0.2 399,119 - +0.4 399,258 - +1.8 399,468 + +0.3 399,627 + BPHYT_RS21575 0.19 +0.8 399,827 + BPHYT_RS21580 0.25 -1.2 400,167 + BPHYT_RS21585 0.25 +1.2 400,314 - BPHYT_RS21585 0.44 +0.2 400,329 + BPHYT_RS21585 0.46 +0.7 400,421 - BPHYT_RS21585 0.58 +1.7 400,710 + -0.7 401,077 + BPHYT_RS21590 0.34 +0.2
Or see this region's nucleotide sequence