Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21195 and BPHYT_RS21200 are separated by 123 nucleotides BPHYT_RS21200 and BPHYT_RS21205 overlap by 7 nucleotides BPHYT_RS21205 and BPHYT_RS21210 are separated by 116 nucleotides
BPHYT_RS21195: BPHYT_RS21195 - hypothetical protein, at 317,496 to 318,581
_RS21195
BPHYT_RS21200: BPHYT_RS21200 - hypothetical protein, at 318,705 to 319,337
_RS21200
BPHYT_RS21205: BPHYT_RS21205 - high-affinity nickel-transporter, at 319,331 to 320,455
_RS21205
BPHYT_RS21210: BPHYT_RS21210 - hypothetical protein, at 320,572 to 321,891
_RS21210
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 318.390 kb on + strand, within BPHYT_RS21195 at 318.484 kb on - strand at 318.584 kb on - strand at 319.014 kb on + strand, within BPHYT_RS21200 at 319.106 kb on + strand, within BPHYT_RS21200 at 319.229 kb on - strand, within BPHYT_RS21200 at 319.304 kb on + strand at 319.766 kb on - strand, within BPHYT_RS21205 at 319.899 kb on + strand, within BPHYT_RS21205 at 319.900 kb on - strand, within BPHYT_RS21205 at 320.050 kb on - strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.211 kb on + strand, within BPHYT_RS21205 at 320.451 kb on + strand at 320.606 kb on + strand at 320.618 kb on + strand at 320.618 kb on + strand at 320.696 kb on + strand at 320.696 kb on + strand at 320.697 kb on - strand at 320.697 kb on - strand at 320.735 kb on + strand, within BPHYT_RS21210 at 320.996 kb on + strand, within BPHYT_RS21210 at 321.208 kb on - strand, within BPHYT_RS21210 at 321.407 kb on - strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.423 kb on - strand, within BPHYT_RS21210
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 318,390 + BPHYT_RS21195 0.82 -0.0 318,484 - -0.5 318,584 - +1.1 319,014 + BPHYT_RS21200 0.49 +0.5 319,106 + BPHYT_RS21200 0.63 +0.6 319,229 - BPHYT_RS21200 0.83 +0.1 319,304 + +0.2 319,766 - BPHYT_RS21205 0.39 -2.8 319,899 + BPHYT_RS21205 0.50 +0.5 319,900 - BPHYT_RS21205 0.51 +0.2 320,050 - BPHYT_RS21205 0.64 -1.6 320,211 + BPHYT_RS21205 0.78 +0.8 320,211 + BPHYT_RS21205 0.78 -0.0 320,451 + -0.2 320,606 + -0.4 320,618 + +0.2 320,618 + -0.9 320,696 + +0.6 320,696 + +0.7 320,697 - -0.9 320,697 - +0.0 320,735 + BPHYT_RS21210 0.12 -0.1 320,996 + BPHYT_RS21210 0.32 -0.3 321,208 - BPHYT_RS21210 0.48 +0.4 321,407 - BPHYT_RS21210 0.63 +0.6 321,422 + BPHYT_RS21210 0.64 +0.6 321,422 + BPHYT_RS21210 0.64 -0.8 321,423 - BPHYT_RS21210 0.64 -1.4
Or see this region's nucleotide sequence