Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21100

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21095 and BPHYT_RS21100 are separated by 45 nucleotidesBPHYT_RS21100 and BPHYT_RS21105 are separated by 27 nucleotides BPHYT_RS21095: BPHYT_RS21095 - omega amino acid--pyruvate aminotransferase, at 293,291 to 294,607 _RS21095 BPHYT_RS21100: BPHYT_RS21100 - methylmalonate-semialdehyde dehydrogenase, at 294,653 to 296,158 _RS21100 BPHYT_RS21105: BPHYT_RS21105 - phenylhydantoinase, at 296,186 to 297,607 _RS21105 Position (kb) 294 295 296 297Strain fitness (log2 ratio) -3 -2 -1 0 1at 294.239 kb on - strand, within BPHYT_RS21095at 294.239 kb on - strand, within BPHYT_RS21095at 294.242 kb on + strand, within BPHYT_RS21095at 294.243 kb on - strand, within BPHYT_RS21095at 294.243 kb on - strand, within BPHYT_RS21095at 296.334 kb on - strand, within BPHYT_RS21105at 296.362 kb on + strand, within BPHYT_RS21105at 296.363 kb on - strand, within BPHYT_RS21105at 296.363 kb on - strand, within BPHYT_RS21105at 296.543 kb on + strand, within BPHYT_RS21105at 297.119 kb on - strand, within BPHYT_RS21105at 297.119 kb on - strand, within BPHYT_RS21105

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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294,239 - BPHYT_RS21095 0.72 -1.8
294,239 - BPHYT_RS21095 0.72 +0.3
294,242 + BPHYT_RS21095 0.72 -2.3
294,243 - BPHYT_RS21095 0.72 -1.2
294,243 - BPHYT_RS21095 0.72 +0.5
296,334 - BPHYT_RS21105 0.10 +0.8
296,362 + BPHYT_RS21105 0.12 +0.3
296,363 - BPHYT_RS21105 0.12 +0.6
296,363 - BPHYT_RS21105 0.12 -0.0
296,543 + BPHYT_RS21105 0.25 -3.0
297,119 - BPHYT_RS21105 0.66 +0.4
297,119 - BPHYT_RS21105 0.66 -0.3

Or see this region's nucleotide sequence