Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21025

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21020 and BPHYT_RS21025 are separated by 329 nucleotides BPHYT_RS21020: BPHYT_RS21020 - branched-chain amino acid ABC transporter substrate-binding protein, at 274,629 to 275,753 _RS21020 BPHYT_RS21025: BPHYT_RS21025 - cation-binding protein, at 276,083 to 276,619 _RS21025 Position (kb) 276 277Strain fitness (log2 ratio) -2 -1 0 1 2at 275.092 kb on - strand, within BPHYT_RS21020at 275.544 kb on + strand, within BPHYT_RS21020at 275.709 kb on + strandat 275.710 kb on - strandat 275.710 kb on - strandat 276.347 kb on + strand, within BPHYT_RS21025at 276.734 kb on - strandat 277.275 kb on + strandat 277.567 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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275,092 - BPHYT_RS21020 0.41 +0.2
275,544 + BPHYT_RS21020 0.81 -2.2
275,709 + +0.1
275,710 - -0.5
275,710 - +0.1
276,347 + BPHYT_RS21025 0.49 +1.3
276,734 - +1.0
277,275 + +2.2
277,567 + -0.4

Or see this region's nucleotide sequence