Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17035 and BPHYT_RS17040 are separated by 26 nucleotides BPHYT_RS17040 and BPHYT_RS17045 are separated by 248 nucleotides BPHYT_RS17045 and BPHYT_RS17050 are separated by 30 nucleotides
BPHYT_RS17035: BPHYT_RS17035 - sugar kinase, at 3,861,612 to 3,862,550
_RS17035
BPHYT_RS17040: BPHYT_RS17040 - membrane protein, at 3,862,577 to 3,863,053
_RS17040
BPHYT_RS17045: BPHYT_RS17045 - histone, at 3,863,302 to 3,863,919
_RS17045
BPHYT_RS17050: BPHYT_RS17050 - hypothetical protein, at 3,863,950 to 3,864,276
_RS17050
Position (kb)
3862
3863
3864 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3861.596 kb on + strand at 3862.047 kb on + strand, within BPHYT_RS17035 at 3862.099 kb on - strand, within BPHYT_RS17035 at 3862.174 kb on - strand, within BPHYT_RS17035 at 3862.178 kb on - strand, within BPHYT_RS17035 at 3862.191 kb on + strand, within BPHYT_RS17035 at 3862.486 kb on - strand at 3862.549 kb on - strand at 3862.646 kb on + strand, within BPHYT_RS17040 at 3862.646 kb on + strand, within BPHYT_RS17040 at 3862.724 kb on - strand, within BPHYT_RS17040 at 3863.230 kb on + strand at 3863.230 kb on + strand at 3863.278 kb on + strand at 3863.279 kb on - strand at 3863.686 kb on - strand, within BPHYT_RS17045 at 3864.037 kb on - strand, within BPHYT_RS17050
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,861,596 + -0.9 3,862,047 + BPHYT_RS17035 0.46 +1.0 3,862,099 - BPHYT_RS17035 0.52 +1.0 3,862,174 - BPHYT_RS17035 0.60 +2.0 3,862,178 - BPHYT_RS17035 0.60 +2.0 3,862,191 + BPHYT_RS17035 0.62 +2.0 3,862,486 - +2.0 3,862,549 - -0.3 3,862,646 + BPHYT_RS17040 0.14 -0.6 3,862,646 + BPHYT_RS17040 0.14 -1.5 3,862,724 - BPHYT_RS17040 0.31 -0.6 3,863,230 + -0.6 3,863,230 + -0.3 3,863,278 + +2.0 3,863,279 - -2.2 3,863,686 - BPHYT_RS17045 0.62 -0.6 3,864,037 - BPHYT_RS17050 0.27 -0.9
Or see this region's nucleotide sequence