Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14395 and BPHYT_RS14400 are separated by 4 nucleotides BPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotides BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides
BPHYT_RS14395: BPHYT_RS14395 - sugar dehydratase, at 3,285,787 to 3,286,830
_RS14395
BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464
_RS14400
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
Position (kb)
3286
3287
3288 Strain fitness (log2 ratio)
-1
0
1
2 at 3286.158 kb on + strand, within BPHYT_RS14395 at 3286.159 kb on - strand, within BPHYT_RS14395 at 3286.165 kb on - strand, within BPHYT_RS14395 at 3286.304 kb on + strand, within BPHYT_RS14395 at 3286.305 kb on - strand, within BPHYT_RS14395 at 3286.386 kb on + strand, within BPHYT_RS14395 at 3286.387 kb on - strand, within BPHYT_RS14395 at 3286.387 kb on - strand, within BPHYT_RS14395 at 3286.499 kb on + strand, within BPHYT_RS14395 at 3286.746 kb on + strand at 3286.801 kb on - strand at 3286.836 kb on + strand at 3286.836 kb on + strand at 3286.930 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.357 kb on - strand, within BPHYT_RS14400 at 3287.479 kb on + strand at 3287.479 kb on + strand at 3287.480 kb on - strand at 3287.480 kb on - strand at 3287.485 kb on + strand at 3287.485 kb on + strand at 3287.486 kb on - strand at 3287.514 kb on - strand at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,286,158 + BPHYT_RS14395 0.36 +1.1 3,286,159 - BPHYT_RS14395 0.36 +2.0 3,286,165 - BPHYT_RS14395 0.36 +1.7 3,286,304 + BPHYT_RS14395 0.50 -0.9 3,286,305 - BPHYT_RS14395 0.50 +1.4 3,286,386 + BPHYT_RS14395 0.57 +1.1 3,286,387 - BPHYT_RS14395 0.57 +2.1 3,286,387 - BPHYT_RS14395 0.57 +1.6 3,286,499 + BPHYT_RS14395 0.68 +2.1 3,286,746 + +1.1 3,286,801 - +2.2 3,286,836 + +1.1 3,286,836 + +0.5 3,286,930 - BPHYT_RS14400 0.15 +0.1 3,287,314 - BPHYT_RS14400 0.76 +2.3 3,287,314 - BPHYT_RS14400 0.76 +2.5 3,287,357 - BPHYT_RS14400 0.83 +2.7 3,287,479 + +2.7 3,287,479 + +1.0 3,287,480 - +0.7 3,287,480 - +1.1 3,287,485 + +2.3 3,287,485 + +2.0 3,287,486 - +1.6 3,287,514 - +1.2 3,288,193 - +0.3 3,288,193 - +0.5 3,288,218 - +0.2 3,288,218 - +0.7 3,288,324 + BPHYT_RS14410 0.20 +2.1
Or see this region's nucleotide sequence