Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14195

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14190 and BPHYT_RS14195 are separated by 220 nucleotidesBPHYT_RS14195 and BPHYT_RS14200 are separated by 493 nucleotides BPHYT_RS14190: BPHYT_RS14190 - carbamoyl phosphate synthase large subunit, at 3,239,695 to 3,242,949 _RS14190 BPHYT_RS14195: BPHYT_RS14195 - carbamoyl phosphate synthase small subunit, at 3,243,170 to 3,244,324 _RS14195 BPHYT_RS14200: BPHYT_RS14200 - membrane protein, at 3,244,818 to 3,246,059 _RS14200 Position (kb) 3243 3244 3245Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3242.379 kb on + strand, within BPHYT_RS14190at 3242.382 kb on - strand, within BPHYT_RS14190at 3242.618 kb on + strand, within BPHYT_RS14190at 3242.619 kb on - strand, within BPHYT_RS14190at 3242.757 kb on - strandat 3242.825 kb on + strandat 3242.826 kb on - strandat 3242.826 kb on - strandat 3242.966 kb on + strandat 3242.966 kb on + strandat 3242.967 kb on - strandat 3242.967 kb on - strandat 3242.967 kb on - strandat 3242.967 kb on - strandat 3243.003 kb on + strandat 3243.003 kb on + strandat 3243.004 kb on - strandat 3243.235 kb on - strandat 3243.565 kb on + strand, within BPHYT_RS14195at 3243.732 kb on + strand, within BPHYT_RS14195at 3243.816 kb on + strand, within BPHYT_RS14195at 3244.100 kb on - strand, within BPHYT_RS14195at 3244.561 kb on - strandat 3244.801 kb on + strandat 3244.802 kb on - strandat 3244.815 kb on - strandat 3244.815 kb on - strandat 3244.880 kb on - strandat 3245.191 kb on + strand, within BPHYT_RS14200at 3245.191 kb on + strandat 3245.192 kb on - strand, within BPHYT_RS14200at 3245.192 kb on - strand, within BPHYT_RS14200at 3245.222 kb on - strand, within BPHYT_RS14200at 3245.232 kb on - strand, within BPHYT_RS14200at 3245.232 kb on - strand, within BPHYT_RS14200

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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3,242,379 + BPHYT_RS14190 0.82 +0.5
3,242,382 - BPHYT_RS14190 0.83 +0.5
3,242,618 + BPHYT_RS14190 0.90 +2.1
3,242,619 - BPHYT_RS14190 0.90 +2.1
3,242,757 - +1.1
3,242,825 + +0.0
3,242,826 - -1.1
3,242,826 - +1.6
3,242,966 + +0.5
3,242,966 + +0.3
3,242,967 - -1.3
3,242,967 - -2.1
3,242,967 - -1.0
3,242,967 - -0.4
3,243,003 + +0.1
3,243,003 + -1.6
3,243,004 - +0.5
3,243,235 - -0.9
3,243,565 + BPHYT_RS14195 0.34 +2.1
3,243,732 + BPHYT_RS14195 0.49 +3.1
3,243,816 + BPHYT_RS14195 0.56 -0.5
3,244,100 - BPHYT_RS14195 0.81 +0.9
3,244,561 - +0.1
3,244,801 + -1.6
3,244,802 - +0.2
3,244,815 - -0.5
3,244,815 - +0.1
3,244,880 - -0.4
3,245,191 + BPHYT_RS14200 0.30 -1.4
3,245,191 + +2.1
3,245,192 - BPHYT_RS14200 0.30 -0.9
3,245,192 - BPHYT_RS14200 0.30 -1.3
3,245,222 - BPHYT_RS14200 0.33 +0.7
3,245,232 - BPHYT_RS14200 0.33 -0.4
3,245,232 - BPHYT_RS14200 0.33 -1.7

Or see this region's nucleotide sequence