Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12740 and BPHYT_RS12745 are separated by 313 nucleotides BPHYT_RS12745 and BPHYT_RS12750 are separated by 122 nucleotides BPHYT_RS12750 and BPHYT_RS12755 are separated by 15 nucleotides BPHYT_RS12755 and BPHYT_RS12760 are separated by 154 nucleotides
BPHYT_RS12740: BPHYT_RS12740 - lysine--tRNA ligase, at 2,905,648 to 2,907,204
_RS12740
BPHYT_RS12745: BPHYT_RS12745 - membrane protein, at 2,907,518 to 2,908,261
_RS12745
BPHYT_RS12750: BPHYT_RS12750 - hypothetical protein, at 2,908,384 to 2,908,581
_RS12750
BPHYT_RS12755: BPHYT_RS12755 - 2Fe-2S type ferredoxin, at 2,908,597 to 2,908,938
_RS12755
BPHYT_RS12760: BPHYT_RS12760 - molecular chaperone HscA, at 2,909,093 to 2,910,964
_RS12760
Position (kb)
2907
2908
2909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2907.397 kb on + strand at 2907.397 kb on + strand at 2907.398 kb on - strand at 2907.398 kb on - strand at 2907.400 kb on - strand at 2907.400 kb on - strand at 2907.480 kb on + strand at 2907.860 kb on + strand, within BPHYT_RS12745 at 2907.860 kb on + strand, within BPHYT_RS12745 at 2907.893 kb on - strand, within BPHYT_RS12745 at 2907.893 kb on - strand, within BPHYT_RS12745 at 2907.896 kb on + strand, within BPHYT_RS12745 at 2907.897 kb on - strand, within BPHYT_RS12745 at 2907.897 kb on - strand, within BPHYT_RS12745 at 2907.897 kb on - strand, within BPHYT_RS12745 at 2907.926 kb on + strand, within BPHYT_RS12745 at 2907.927 kb on - strand, within BPHYT_RS12745 at 2908.190 kb on + strand at 2908.190 kb on + strand at 2908.190 kb on + strand at 2908.191 kb on - strand at 2908.191 kb on - strand at 2908.191 kb on - strand at 2908.995 kb on - strand at 2909.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,907,397 + -0.7 2,907,397 + +0.8 2,907,398 - +0.4 2,907,398 - -0.7 2,907,400 - +0.3 2,907,400 - -1.5 2,907,480 + -0.0 2,907,860 + BPHYT_RS12745 0.46 -0.4 2,907,860 + BPHYT_RS12745 0.46 +1.8 2,907,893 - BPHYT_RS12745 0.50 -1.5 2,907,893 - BPHYT_RS12745 0.50 +0.4 2,907,896 + BPHYT_RS12745 0.51 +0.1 2,907,897 - BPHYT_RS12745 0.51 -0.1 2,907,897 - BPHYT_RS12745 0.51 +0.0 2,907,897 - BPHYT_RS12745 0.51 -2.8 2,907,926 + BPHYT_RS12745 0.55 -1.0 2,907,927 - BPHYT_RS12745 0.55 -0.0 2,908,190 + +1.5 2,908,190 + -2.5 2,908,190 + +1.0 2,908,191 - -0.3 2,908,191 - +0.0 2,908,191 - -0.7 2,908,995 - +0.3 2,909,017 - +2.0
Or see this region's nucleotide sequence