Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12180 and BPHYT_RS12185 are separated by 682 nucleotides BPHYT_RS12185 and BPHYT_RS12190 are separated by 70 nucleotides BPHYT_RS12190 and BPHYT_RS12195 overlap by 1 nucleotides
BPHYT_RS12180: BPHYT_RS12180 - multidrug DMT transporter permease, at 2,781,072 to 2,782,067
_RS12180
BPHYT_RS12185: BPHYT_RS12185 - succinyldiaminopimelate aminotransferase, at 2,782,750 to 2,783,967
_RS12185
BPHYT_RS12190: BPHYT_RS12190 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,784,038 to 2,784,865
_RS12190
BPHYT_RS12195: BPHYT_RS12195 - arsenate reductase, at 2,784,865 to 2,785,230
_RS12195
Position (kb)
2782
2783
2784 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2781.766 kb on + strand, within BPHYT_RS12180 at 2781.767 kb on - strand, within BPHYT_RS12180 at 2781.813 kb on + strand, within BPHYT_RS12180 at 2781.813 kb on + strand, within BPHYT_RS12180 at 2781.814 kb on - strand, within BPHYT_RS12180 at 2781.991 kb on + strand at 2782.374 kb on + strand at 2782.374 kb on + strand at 2782.374 kb on + strand at 2782.493 kb on + strand at 2782.494 kb on - strand at 2782.494 kb on - strand at 2782.591 kb on + strand at 2782.592 kb on - strand at 2782.619 kb on + strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.620 kb on - strand at 2782.621 kb on + strand at 2782.622 kb on - strand at 2782.622 kb on - strand at 2782.842 kb on + strand at 2782.842 kb on + strand at 2782.994 kb on - strand, within BPHYT_RS12185 at 2782.994 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.121 kb on - strand, within BPHYT_RS12185 at 2783.140 kb on + strand, within BPHYT_RS12185 at 2783.150 kb on - strand, within BPHYT_RS12185 at 2783.185 kb on + strand, within BPHYT_RS12185 at 2783.185 kb on + strand, within BPHYT_RS12185 at 2783.196 kb on + strand, within BPHYT_RS12185 at 2783.196 kb on + strand, within BPHYT_RS12185 at 2783.272 kb on + strand, within BPHYT_RS12185 at 2783.272 kb on + strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.273 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.565 kb on - strand, within BPHYT_RS12185 at 2783.575 kb on + strand, within BPHYT_RS12185 at 2783.576 kb on - strand, within BPHYT_RS12185 at 2783.576 kb on - strand, within BPHYT_RS12185 at 2783.740 kb on + strand, within BPHYT_RS12185 at 2783.740 kb on + strand, within BPHYT_RS12185 at 2783.811 kb on + strand, within BPHYT_RS12185 at 2783.811 kb on + strand, within BPHYT_RS12185 at 2784.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,781,766 + BPHYT_RS12180 0.70 +0.4 2,781,767 - BPHYT_RS12180 0.70 +1.0 2,781,813 + BPHYT_RS12180 0.74 -0.5 2,781,813 + BPHYT_RS12180 0.74 -0.1 2,781,814 - BPHYT_RS12180 0.74 +0.0 2,781,991 + -0.6 2,782,374 + -1.0 2,782,374 + -2.2 2,782,374 + -1.6 2,782,493 + -0.2 2,782,494 - -0.6 2,782,494 - +0.7 2,782,591 + -1.5 2,782,592 - +0.5 2,782,619 + -0.4 2,782,620 - -0.5 2,782,620 - +0.1 2,782,620 - -0.8 2,782,620 - -1.3 2,782,620 - +0.8 2,782,621 + -0.3 2,782,622 - -0.9 2,782,622 - -0.4 2,782,842 + +0.0 2,782,842 + -0.0 2,782,994 - BPHYT_RS12185 0.20 +0.6 2,782,994 - BPHYT_RS12185 0.20 -0.4 2,783,121 - BPHYT_RS12185 0.30 -0.9 2,783,121 - BPHYT_RS12185 0.30 +1.3 2,783,121 - BPHYT_RS12185 0.30 -0.7 2,783,140 + BPHYT_RS12185 0.32 -0.0 2,783,150 - BPHYT_RS12185 0.33 +1.4 2,783,185 + BPHYT_RS12185 0.36 +2.2 2,783,185 + BPHYT_RS12185 0.36 +4.4 2,783,196 + BPHYT_RS12185 0.37 +0.4 2,783,196 + BPHYT_RS12185 0.37 -0.0 2,783,272 + BPHYT_RS12185 0.43 +0.7 2,783,272 + BPHYT_RS12185 0.43 -1.3 2,783,273 - BPHYT_RS12185 0.43 +0.4 2,783,273 - BPHYT_RS12185 0.43 +1.1 2,783,273 - BPHYT_RS12185 0.43 +0.4 2,783,273 - BPHYT_RS12185 0.43 -0.3 2,783,273 - BPHYT_RS12185 0.43 +1.4 2,783,273 - BPHYT_RS12185 0.43 -0.6 2,783,565 - BPHYT_RS12185 0.67 -0.2 2,783,565 - BPHYT_RS12185 0.67 -1.8 2,783,565 - BPHYT_RS12185 0.67 +0.2 2,783,575 + BPHYT_RS12185 0.68 +1.4 2,783,576 - BPHYT_RS12185 0.68 -2.8 2,783,576 - BPHYT_RS12185 0.68 -0.3 2,783,740 + BPHYT_RS12185 0.81 +0.6 2,783,740 + BPHYT_RS12185 0.81 -1.6 2,783,811 + BPHYT_RS12185 0.87 -0.0 2,783,811 + BPHYT_RS12185 0.87 +2.6 2,784,037 + +0.6
Or see this region's nucleotide sequence