Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10125 and BPHYT_RS10130 are separated by 711 nucleotides BPHYT_RS10130 and BPHYT_RS10135 are separated by 35 nucleotides BPHYT_RS10135 and BPHYT_RS10140 are separated by 210 nucleotides
BPHYT_RS10125: BPHYT_RS10125 - hypothetical protein, at 2,305,909 to 2,306,361
_RS10125
BPHYT_RS10130: BPHYT_RS10130 - hypothetical protein, at 2,307,073 to 2,307,252
_RS10130
BPHYT_RS10135: BPHYT_RS10135 - hypothetical protein, at 2,307,288 to 2,307,713
_RS10135
BPHYT_RS10140: BPHYT_RS10140 - ABC transporter substrate-binding protein, at 2,307,924 to 2,308,706
_RS10140
Position (kb)
2307
2308 Strain fitness (log2 ratio)
-2
-1
0
1 at 2306.478 kb on - strand at 2306.478 kb on - strand at 2306.702 kb on - strand at 2306.846 kb on + strand at 2307.012 kb on + strand at 2307.031 kb on - strand at 2307.119 kb on + strand, within BPHYT_RS10130 at 2307.247 kb on - strand at 2307.520 kb on + strand, within BPHYT_RS10135 at 2307.742 kb on - strand at 2307.795 kb on - strand at 2307.795 kb on - strand at 2307.796 kb on + strand at 2307.796 kb on + strand at 2307.796 kb on + strand at 2307.797 kb on - strand at 2307.935 kb on - strand at 2307.974 kb on - strand at 2308.267 kb on + strand, within BPHYT_RS10140 at 2308.268 kb on - strand, within BPHYT_RS10140 at 2308.268 kb on - strand, within BPHYT_RS10140
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,306,478 - -0.3 2,306,478 - +0.3 2,306,702 - -2.0 2,306,846 + -0.5 2,307,012 + +1.0 2,307,031 - -0.9 2,307,119 + BPHYT_RS10130 0.26 +0.6 2,307,247 - +0.2 2,307,520 + BPHYT_RS10135 0.54 -1.5 2,307,742 - -2.5 2,307,795 - +0.6 2,307,795 - +0.5 2,307,796 + -0.7 2,307,796 + -0.2 2,307,796 + -0.9 2,307,797 - -0.0 2,307,935 - +0.1 2,307,974 - +0.4 2,308,267 + BPHYT_RS10140 0.44 -0.6 2,308,268 - BPHYT_RS10140 0.44 +0.5 2,308,268 - BPHYT_RS10140 0.44 -0.2
Or see this region's nucleotide sequence