Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07925 and BPHYT_RS07930 are separated by 330 nucleotides BPHYT_RS07930 and BPHYT_RS07935 are separated by 87 nucleotides BPHYT_RS07935 and BPHYT_RS07940 are separated by 144 nucleotides
BPHYT_RS07925: BPHYT_RS07925 - glutamine amidotransferase, at 1,804,396 to 1,804,977
_RS07925
BPHYT_RS07930: BPHYT_RS07930 - hypothetical protein, at 1,805,308 to 1,805,754
_RS07930
BPHYT_RS07935: BPHYT_RS07935 - Rrf2 family transcriptional regulator, at 1,805,842 to 1,806,297
_RS07935
BPHYT_RS07940: BPHYT_RS07940 - 3-beta hydroxysteroid dehydrogenase, at 1,806,442 to 1,807,089
_RS07940
Position (kb) 1805
1806 Strain fitness (log2 ratio) -1
0
1 at 1804.697 kb on + strand, within BPHYT_RS07925 at 1805.044 kb on + strand at 1805.045 kb on - strand at 1805.295 kb on - strand at 1805.449 kb on + strand, within BPHYT_RS07930 at 1805.449 kb on + strand, within BPHYT_RS07930 at 1805.449 kb on + strand, within BPHYT_RS07930 at 1805.450 kb on - strand, within BPHYT_RS07930 at 1805.450 kb on - strand, within BPHYT_RS07930 at 1806.076 kb on + strand, within BPHYT_RS07935 at 1806.301 kb on - strand at 1806.314 kb on + strand at 1806.315 kb on - strand at 1806.665 kb on - strand, within BPHYT_RS07940 at 1806.665 kb on - strand, within BPHYT_RS07940
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,804,697 + BPHYT_RS07925 0.52 +0.0 1,805,044 + -1.0 1,805,045 - -0.9 1,805,295 - -0.3 1,805,449 + BPHYT_RS07930 0.32 -1.1 1,805,449 + BPHYT_RS07930 0.32 -0.9 1,805,449 + BPHYT_RS07930 0.32 +0.5 1,805,450 - BPHYT_RS07930 0.32 -0.4 1,805,450 - BPHYT_RS07930 0.32 -1.0 1,806,076 + BPHYT_RS07935 0.51 +0.4 1,806,301 - +0.3 1,806,314 + +0.3 1,806,315 - +0.2 1,806,665 - BPHYT_RS07940 0.34 -0.8 1,806,665 - BPHYT_RS07940 0.34 -0.5
Or see this region's nucleotide sequence