Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06380 and BPHYT_RS06385 are separated by 369 nucleotides BPHYT_RS06385 and BPHYT_RS06390 are separated by 74 nucleotides BPHYT_RS06390 and BPHYT_RS06395 are separated by 5 nucleotides BPHYT_RS06395 and BPHYT_RS06400 are separated by 89 nucleotides
BPHYT_RS06380: BPHYT_RS06380 - septum formation protein Maf, at 1,453,460 to 1,454,089
_RS06380
BPHYT_RS06385: BPHYT_RS06385 - ribosomal RNA large subunit methyltransferase H, at 1,454,459 to 1,454,929
_RS06385
BPHYT_RS06390: BPHYT_RS06390 - ribosome-associated protein IOJAP, at 1,455,004 to 1,455,468
_RS06390
BPHYT_RS06395: BPHYT_RS06395 - nicotinic acid mononucleotide adenylyltransferase, at 1,455,474 to 1,456,145
_RS06395
BPHYT_RS06400: BPHYT_RS06400 - oxygen-dependent coproporphyrinogen-III oxidase, at 1,456,235 to 1,457,158
_RS06400
Position (kb)
1455
1456 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1454.053 kb on - strand at 1454.218 kb on + strand at 1454.274 kb on - strand at 1454.313 kb on + strand at 1454.313 kb on + strand at 1454.314 kb on - strand at 1454.472 kb on + strand at 1454.862 kb on + strand, within BPHYT_RS06385 at 1454.862 kb on + strand, within BPHYT_RS06385 at 1454.863 kb on - strand, within BPHYT_RS06385 at 1454.918 kb on - strand at 1455.005 kb on + strand at 1455.005 kb on + strand at 1455.005 kb on + strand at 1455.005 kb on + strand at 1455.006 kb on - strand at 1455.006 kb on - strand at 1455.176 kb on + strand, within BPHYT_RS06390 at 1455.176 kb on + strand, within BPHYT_RS06390 at 1455.177 kb on - strand, within BPHYT_RS06390 at 1455.177 kb on - strand, within BPHYT_RS06390
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,454,053 - +0.3 1,454,218 + -0.7 1,454,274 - +1.0 1,454,313 + -0.2 1,454,313 + -1.1 1,454,314 - -0.8 1,454,472 + -0.4 1,454,862 + BPHYT_RS06385 0.86 +1.3 1,454,862 + BPHYT_RS06385 0.86 -0.1 1,454,863 - BPHYT_RS06385 0.86 +0.5 1,454,918 - -0.2 1,455,005 + +3.4 1,455,005 + +0.3 1,455,005 + +0.6 1,455,005 + +0.8 1,455,006 - +0.1 1,455,006 - +0.2 1,455,176 + BPHYT_RS06390 0.37 +0.8 1,455,176 + BPHYT_RS06390 0.37 +1.0 1,455,177 - BPHYT_RS06390 0.37 +1.0 1,455,177 - BPHYT_RS06390 0.37 +1.3
Or see this region's nucleotide sequence