Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05260 and BPHYT_RS05265 are separated by 106 nucleotides BPHYT_RS05265 and BPHYT_RS05270 are separated by 159 nucleotides BPHYT_RS05270 and BPHYT_RS05275 are separated by 302 nucleotides
BPHYT_RS05260: BPHYT_RS05260 - hypothetical protein, at 1,214,475 to 1,215,032
_RS05260
BPHYT_RS05265: BPHYT_RS05265 - hypothetical protein, at 1,215,139 to 1,215,414
_RS05265
BPHYT_RS05270: BPHYT_RS05270 - hypothetical protein, at 1,215,574 to 1,216,323
_RS05270
BPHYT_RS05275: BPHYT_RS05275 - integrase, at 1,216,626 to 1,217,501
_RS05275
Position (kb)
1215
1216
1217 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1214.580 kb on + strand, within BPHYT_RS05260 at 1214.580 kb on + strand, within BPHYT_RS05260 at 1214.655 kb on + strand, within BPHYT_RS05260 at 1214.664 kb on + strand, within BPHYT_RS05260 at 1214.664 kb on + strand, within BPHYT_RS05260 at 1214.665 kb on - strand, within BPHYT_RS05260 at 1214.665 kb on - strand, within BPHYT_RS05260 at 1214.665 kb on - strand, within BPHYT_RS05260 at 1214.719 kb on - strand, within BPHYT_RS05260 at 1214.907 kb on - strand, within BPHYT_RS05260 at 1214.924 kb on + strand, within BPHYT_RS05260 at 1215.033 kb on - strand at 1215.178 kb on + strand, within BPHYT_RS05265 at 1215.179 kb on - strand, within BPHYT_RS05265 at 1215.183 kb on - strand, within BPHYT_RS05265 at 1215.183 kb on - strand, within BPHYT_RS05265 at 1215.215 kb on + strand, within BPHYT_RS05265 at 1215.221 kb on + strand, within BPHYT_RS05265 at 1215.221 kb on + strand, within BPHYT_RS05265 at 1215.222 kb on - strand, within BPHYT_RS05265 at 1215.277 kb on + strand, within BPHYT_RS05265 at 1215.278 kb on - strand, within BPHYT_RS05265 at 1215.278 kb on - strand, within BPHYT_RS05265 at 1215.278 kb on - strand, within BPHYT_RS05265 at 1215.436 kb on + strand at 1215.463 kb on + strand at 1215.463 kb on + strand at 1215.463 kb on + strand at 1215.463 kb on + strand at 1215.464 kb on - strand at 1215.539 kb on + strand at 1215.582 kb on + strand at 1215.688 kb on + strand, within BPHYT_RS05270 at 1215.689 kb on - strand, within BPHYT_RS05270 at 1215.873 kb on + strand, within BPHYT_RS05270 at 1215.873 kb on + strand, within BPHYT_RS05270 at 1215.913 kb on + strand, within BPHYT_RS05270 at 1215.913 kb on + strand, within BPHYT_RS05270 at 1215.914 kb on - strand, within BPHYT_RS05270 at 1216.056 kb on + strand, within BPHYT_RS05270 at 1216.060 kb on + strand, within BPHYT_RS05270 at 1216.060 kb on + strand, within BPHYT_RS05270 at 1216.060 kb on + strand, within BPHYT_RS05270 at 1216.061 kb on - strand, within BPHYT_RS05270 at 1216.067 kb on + strand, within BPHYT_RS05270 at 1216.077 kb on - strand, within BPHYT_RS05270 at 1216.077 kb on - strand, within BPHYT_RS05270 at 1216.077 kb on - strand, within BPHYT_RS05270 at 1216.302 kb on + strand at 1216.397 kb on - strand at 1216.397 kb on - strand at 1216.419 kb on + strand at 1216.436 kb on - strand at 1216.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,214,580 + BPHYT_RS05260 0.19 -0.8 1,214,580 + BPHYT_RS05260 0.19 -2.1 1,214,655 + BPHYT_RS05260 0.32 -0.6 1,214,664 + BPHYT_RS05260 0.34 +2.4 1,214,664 + BPHYT_RS05260 0.34 -0.2 1,214,665 - BPHYT_RS05260 0.34 +0.4 1,214,665 - BPHYT_RS05260 0.34 -0.2 1,214,665 - BPHYT_RS05260 0.34 -0.2 1,214,719 - BPHYT_RS05260 0.44 -1.3 1,214,907 - BPHYT_RS05260 0.77 +0.1 1,214,924 + BPHYT_RS05260 0.80 +0.1 1,215,033 - -0.1 1,215,178 + BPHYT_RS05265 0.14 -0.0 1,215,179 - BPHYT_RS05265 0.14 -0.7 1,215,183 - BPHYT_RS05265 0.16 -1.3 1,215,183 - BPHYT_RS05265 0.16 +1.2 1,215,215 + BPHYT_RS05265 0.28 -0.6 1,215,221 + BPHYT_RS05265 0.30 +0.1 1,215,221 + BPHYT_RS05265 0.30 -1.5 1,215,222 - BPHYT_RS05265 0.30 +0.2 1,215,277 + BPHYT_RS05265 0.50 -0.5 1,215,278 - BPHYT_RS05265 0.50 -0.4 1,215,278 - BPHYT_RS05265 0.50 -0.4 1,215,278 - BPHYT_RS05265 0.50 -1.1 1,215,436 + -0.6 1,215,463 + +0.2 1,215,463 + -1.3 1,215,463 + -0.1 1,215,463 + -2.2 1,215,464 - +0.6 1,215,539 + -0.3 1,215,582 + -0.3 1,215,688 + BPHYT_RS05270 0.15 -0.2 1,215,689 - BPHYT_RS05270 0.15 -0.8 1,215,873 + BPHYT_RS05270 0.40 +0.7 1,215,873 + BPHYT_RS05270 0.40 +0.5 1,215,913 + BPHYT_RS05270 0.45 -0.2 1,215,913 + BPHYT_RS05270 0.45 +0.7 1,215,914 - BPHYT_RS05270 0.45 -0.4 1,216,056 + BPHYT_RS05270 0.64 +0.1 1,216,060 + BPHYT_RS05270 0.65 +1.1 1,216,060 + BPHYT_RS05270 0.65 +0.5 1,216,060 + BPHYT_RS05270 0.65 +0.1 1,216,061 - BPHYT_RS05270 0.65 -0.5 1,216,067 + BPHYT_RS05270 0.66 +0.4 1,216,077 - BPHYT_RS05270 0.67 +0.9 1,216,077 - BPHYT_RS05270 0.67 +2.6 1,216,077 - BPHYT_RS05270 0.67 -0.1 1,216,302 + +0.5 1,216,397 - -0.5 1,216,397 - +0.2 1,216,419 + +0.0 1,216,436 - -1.3 1,216,460 - +0.3
Or see this region's nucleotide sequence