Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04370

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04365 and BPHYT_RS04370 are separated by 377 nucleotidesBPHYT_RS04370 and BPHYT_RS04375 overlap by 4 nucleotidesBPHYT_RS04375 and BPHYT_RS04380 are separated by 7 nucleotides BPHYT_RS04365: BPHYT_RS04365 - membrane protein, at 1,004,981 to 1,006,405 _RS04365 BPHYT_RS04370: BPHYT_RS04370 - glycosyl transferase, at 1,006,783 to 1,007,628 _RS04370 BPHYT_RS04375: BPHYT_RS04375 - epimerase, at 1,007,625 to 1,008,581 _RS04375 BPHYT_RS04380: BPHYT_RS04380 - glycosyl transferase, at 1,008,589 to 1,009,620 _RS04380 Position (kb) 1006 1007 1008Strain fitness (log2 ratio) -2 -1 0 1 2at 1005.939 kb on + strand, within BPHYT_RS04365at 1005.940 kb on - strand, within BPHYT_RS04365at 1005.940 kb on - strand, within BPHYT_RS04365at 1005.940 kb on - strand, within BPHYT_RS04365at 1006.030 kb on + strand, within BPHYT_RS04365at 1006.031 kb on - strand, within BPHYT_RS04365at 1006.299 kb on + strandat 1006.299 kb on + strandat 1006.326 kb on + strandat 1006.520 kb on - strandat 1006.524 kb on + strandat 1006.552 kb on + strandat 1006.553 kb on - strandat 1006.600 kb on - strandat 1006.602 kb on + strandat 1006.603 kb on - strandat 1006.603 kb on - strandat 1006.633 kb on + strandat 1006.634 kb on - strandat 1006.671 kb on + strandat 1006.765 kb on + strandat 1006.765 kb on + strandat 1006.780 kb on + strandat 1007.079 kb on + strand, within BPHYT_RS04370at 1007.566 kb on - strandat 1007.624 kb on + strandat 1007.624 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,005,939 + BPHYT_RS04365 0.67 +0.6
1,005,940 - BPHYT_RS04365 0.67 -0.2
1,005,940 - BPHYT_RS04365 0.67 +1.9
1,005,940 - BPHYT_RS04365 0.67 +0.3
1,006,030 + BPHYT_RS04365 0.74 -0.1
1,006,031 - BPHYT_RS04365 0.74 -0.6
1,006,299 + +0.5
1,006,299 + -1.1
1,006,326 + +0.8
1,006,520 - -0.3
1,006,524 + -0.6
1,006,552 + -0.7
1,006,553 - -0.1
1,006,600 - +0.7
1,006,602 + -0.4
1,006,603 - +0.4
1,006,603 - -0.5
1,006,633 + -1.0
1,006,634 - -0.3
1,006,671 + -2.4
1,006,765 + +0.6
1,006,765 + +0.4
1,006,780 + +0.4
1,007,079 + BPHYT_RS04370 0.35 +2.0
1,007,566 - +2.0
1,007,624 + +0.7
1,007,624 + -0.3

Or see this region's nucleotide sequence