Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02895 and BPHYT_RS02900 are separated by 337 nucleotides BPHYT_RS02900 and BPHYT_RS02905 are separated by 170 nucleotides BPHYT_RS02905 and BPHYT_RS02910 are separated by 187 nucleotides BPHYT_RS02910 and BPHYT_RS02915 overlap by 4 nucleotides
BPHYT_RS02895: BPHYT_RS02895 - PTS sugar transporter subunit IIA, at 645,072 to 645,527
_RS02895
BPHYT_RS02900: BPHYT_RS02900 - ribosome hibernation promoting factor, at 645,865 to 646,221
_RS02900
BPHYT_RS02905: BPHYT_RS02905 - RNA polymerase subunit sigma-54, at 646,392 to 647,903
_RS02905
BPHYT_RS02910: BPHYT_RS02910 - ABC transporter, at 648,091 to 648,876
_RS02910
BPHYT_RS02915: BPHYT_RS02915 - organic solvent tolerance protein OstA, at 648,873 to 649,544
_RS02915
Position (kb) 646
647
648 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 645.663 kb on + strand at 645.691 kb on + strand at 645.691 kb on + strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.693 kb on + strand at 645.693 kb on + strand at 645.693 kb on + strand at 645.694 kb on - strand at 645.694 kb on - strand at 645.736 kb on + strand at 645.884 kb on - strand at 645.902 kb on + strand, within BPHYT_RS02900 at 645.903 kb on - strand, within BPHYT_RS02900 at 645.996 kb on - strand, within BPHYT_RS02900 at 646.139 kb on - strand, within BPHYT_RS02900 at 646.186 kb on + strand at 646.221 kb on + strand at 646.221 kb on + strand at 646.237 kb on + strand at 646.237 kb on + strand at 646.238 kb on - strand at 646.238 kb on - strand at 646.265 kb on + strand at 646.266 kb on - strand at 646.266 kb on - strand at 646.266 kb on - strand at 646.365 kb on + strand at 646.393 kb on + strand at 646.394 kb on - strand at 646.442 kb on - strand at 646.455 kb on - strand at 646.540 kb on - strand at 646.553 kb on + strand, within BPHYT_RS02905 at 646.554 kb on - strand, within BPHYT_RS02905 at 646.621 kb on + strand, within BPHYT_RS02905 at 646.625 kb on + strand, within BPHYT_RS02905 at 646.626 kb on - strand, within BPHYT_RS02905 at 646.657 kb on + strand, within BPHYT_RS02905 at 646.657 kb on + strand at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.706 kb on + strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.895 kb on + strand, within BPHYT_RS02905 at 646.916 kb on - strand, within BPHYT_RS02905 at 646.916 kb on - strand, within BPHYT_RS02905 at 646.925 kb on + strand, within BPHYT_RS02905 at 646.929 kb on - strand, within BPHYT_RS02905 at 646.929 kb on - strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.012 kb on - strand, within BPHYT_RS02905 at 647.012 kb on - strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.172 kb on + strand, within BPHYT_RS02905 at 647.189 kb on + strand, within BPHYT_RS02905 at 647.270 kb on + strand, within BPHYT_RS02905 at 647.270 kb on + strand, within BPHYT_RS02905 at 647.271 kb on - strand, within BPHYT_RS02905 at 647.271 kb on - strand, within BPHYT_RS02905 at 647.365 kb on + strand, within BPHYT_RS02905 at 647.366 kb on - strand, within BPHYT_RS02905 at 647.536 kb on + strand, within BPHYT_RS02905 at 647.593 kb on + strand, within BPHYT_RS02905 at 647.594 kb on - strand, within BPHYT_RS02905 at 647.654 kb on + strand, within BPHYT_RS02905 at 647.654 kb on + strand, within BPHYT_RS02905 at 647.655 kb on - strand, within BPHYT_RS02905 at 647.655 kb on - strand, within BPHYT_RS02905 at 647.816 kb on + strand at 647.816 kb on + strand at 647.817 kb on - strand at 647.817 kb on - strand at 647.826 kb on + strand at 647.826 kb on + strand at 647.826 kb on + strand at 647.827 kb on - strand at 647.827 kb on - strand at 647.827 kb on - strand at 647.923 kb on + strand at 647.947 kb on - strand at 647.966 kb on + strand at 647.966 kb on + strand at 648.092 kb on + strand at 648.092 kb on + strand at 648.092 kb on + strand at 648.092 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 645,663 + +3.6 645,691 + -0.2 645,691 + +0.6 645,692 - +0.1 645,692 - -0.6 645,692 - -1.2 645,692 - +0.4 645,693 + +1.3 645,693 + +0.7 645,693 + +2.8 645,694 - -1.5 645,694 - +0.4 645,736 + -0.7 645,884 - +0.5 645,902 + BPHYT_RS02900 0.10 -0.3 645,903 - BPHYT_RS02900 0.11 +1.9 645,996 - BPHYT_RS02900 0.37 -0.6 646,139 - BPHYT_RS02900 0.77 +0.6 646,186 + -0.7 646,221 + -1.7 646,221 + +0.0 646,237 + -0.4 646,237 + -0.4 646,238 - -1.1 646,238 - +0.4 646,265 + -0.9 646,266 - +0.9 646,266 - +1.5 646,266 - -0.1 646,365 + -0.2 646,393 + -1.3 646,394 - +2.6 646,442 - +2.8 646,455 - +2.0 646,540 - +2.2 646,553 + BPHYT_RS02905 0.11 +3.4 646,554 - BPHYT_RS02905 0.11 +2.1 646,621 + BPHYT_RS02905 0.15 +2.4 646,625 + BPHYT_RS02905 0.15 +2.6 646,626 - BPHYT_RS02905 0.15 +2.1 646,657 + BPHYT_RS02905 0.18 +2.3 646,657 + +4.3 646,658 - BPHYT_RS02905 0.18 +2.1 646,658 - BPHYT_RS02905 0.18 +2.2 646,658 - BPHYT_RS02905 0.18 +2.0 646,658 - BPHYT_RS02905 0.18 +2.2 646,706 + BPHYT_RS02905 0.21 +1.9 646,707 - BPHYT_RS02905 0.21 +2.3 646,707 - BPHYT_RS02905 0.21 +2.8 646,707 - BPHYT_RS02905 0.21 +2.3 646,895 + BPHYT_RS02905 0.33 +2.4 646,916 - BPHYT_RS02905 0.35 +2.5 646,916 - BPHYT_RS02905 0.35 +2.4 646,925 + BPHYT_RS02905 0.35 +2.6 646,929 - BPHYT_RS02905 0.36 +2.2 646,929 - BPHYT_RS02905 0.36 +2.0 647,011 + BPHYT_RS02905 0.41 +2.8 647,011 + BPHYT_RS02905 0.41 +2.2 647,011 + BPHYT_RS02905 0.41 +2.5 647,011 + BPHYT_RS02905 0.41 +2.1 647,011 + BPHYT_RS02905 0.41 +2.3 647,012 - BPHYT_RS02905 0.41 +2.2 647,012 - BPHYT_RS02905 0.41 +2.1 647,070 + BPHYT_RS02905 0.45 +2.7 647,070 + BPHYT_RS02905 0.45 +2.9 647,070 + BPHYT_RS02905 0.45 +2.4 647,070 + BPHYT_RS02905 0.45 +2.2 647,071 - BPHYT_RS02905 0.45 +2.6 647,071 - BPHYT_RS02905 0.45 +2.2 647,071 - BPHYT_RS02905 0.45 +2.3 647,071 - BPHYT_RS02905 0.45 +2.3 647,071 - BPHYT_RS02905 0.45 +2.1 647,071 - BPHYT_RS02905 0.45 +2.1 647,172 + BPHYT_RS02905 0.52 +2.3 647,189 + BPHYT_RS02905 0.53 +3.4 647,270 + BPHYT_RS02905 0.58 +2.6 647,270 + BPHYT_RS02905 0.58 +2.2 647,271 - BPHYT_RS02905 0.58 +2.7 647,271 - BPHYT_RS02905 0.58 +2.3 647,365 + BPHYT_RS02905 0.64 +2.2 647,366 - BPHYT_RS02905 0.64 +2.3 647,536 + BPHYT_RS02905 0.76 +1.9 647,593 + BPHYT_RS02905 0.79 +2.4 647,594 - BPHYT_RS02905 0.79 +2.2 647,654 + BPHYT_RS02905 0.83 +2.2 647,654 + BPHYT_RS02905 0.83 +2.7 647,655 - BPHYT_RS02905 0.84 +2.2 647,655 - BPHYT_RS02905 0.84 +2.1 647,816 + +2.2 647,816 + +2.1 647,817 - +2.1 647,817 - +2.4 647,826 + +1.3 647,826 + +2.2 647,826 + +2.1 647,827 - +3.0 647,827 - +2.2 647,827 - +2.1 647,923 + +0.9 647,947 - -1.2 647,966 + -0.4 647,966 + +1.8 648,092 + -0.9 648,092 + -0.6 648,092 + -1.1 648,092 + -0.6
Or see this region's nucleotide sequence