Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02330 and BPHYT_RS02335 are separated by 128 nucleotides BPHYT_RS02335 and BPHYT_RS02340 are separated by 253 nucleotides
BPHYT_RS02330: BPHYT_RS02330 - IclR family transcriptional regulator, at 534,240 to 535,142
_RS02330
BPHYT_RS02335: BPHYT_RS02335 - fumarylacetoacetate hydrolase, at 535,271 to 536,254
_RS02335
BPHYT_RS02340: BPHYT_RS02340 - branched-chain amino acid ABC transporter substrate-binding protein, at 536,508 to 537,743
_RS02340
Position (kb) 535
536
537 Strain fitness (log2 ratio) -2
-1
0
1 at 534.277 kb on + strand at 534.277 kb on + strand at 534.278 kb on - strand at 534.335 kb on + strand, within BPHYT_RS02330 at 534.586 kb on + strand, within BPHYT_RS02330 at 534.587 kb on - strand, within BPHYT_RS02330 at 534.895 kb on + strand, within BPHYT_RS02330 at 534.896 kb on - strand, within BPHYT_RS02330 at 535.178 kb on + strand at 535.178 kb on + strand at 535.179 kb on - strand at 535.179 kb on - strand at 535.179 kb on - strand at 535.227 kb on + strand at 535.227 kb on + strand at 535.228 kb on - strand at 535.233 kb on - strand at 535.233 kb on - strand at 535.252 kb on + strand at 535.532 kb on + strand, within BPHYT_RS02335 at 535.532 kb on + strand, within BPHYT_RS02335 at 535.532 kb on + strand, within BPHYT_RS02335 at 535.554 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.653 kb on + strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 535.654 kb on - strand, within BPHYT_RS02335 at 536.094 kb on - strand, within BPHYT_RS02335 at 536.173 kb on + strand at 536.174 kb on - strand at 536.297 kb on + strand at 536.361 kb on - strand at 536.475 kb on - strand at 536.635 kb on + strand, within BPHYT_RS02340 at 536.636 kb on - strand, within BPHYT_RS02340 at 536.636 kb on - strand, within BPHYT_RS02340 at 536.677 kb on + strand, within BPHYT_RS02340 at 536.678 kb on - strand, within BPHYT_RS02340 at 536.773 kb on - strand, within BPHYT_RS02340 at 536.801 kb on + strand, within BPHYT_RS02340 at 536.802 kb on - strand, within BPHYT_RS02340 at 536.903 kb on - strand, within BPHYT_RS02340 at 537.105 kb on + strand, within BPHYT_RS02340 at 537.105 kb on + strand, within BPHYT_RS02340 at 537.203 kb on + strand, within BPHYT_RS02340 at 537.203 kb on + strand, within BPHYT_RS02340
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 534,277 + +0.2 534,277 + -1.5 534,278 - +0.5 534,335 + BPHYT_RS02330 0.11 +0.8 534,586 + BPHYT_RS02330 0.38 -1.7 534,587 - BPHYT_RS02330 0.38 -2.0 534,895 + BPHYT_RS02330 0.73 -1.5 534,896 - BPHYT_RS02330 0.73 -1.2 535,178 + -0.3 535,178 + -0.9 535,179 - -0.7 535,179 - -1.0 535,179 - -0.5 535,227 + -1.0 535,227 + -0.1 535,228 - -0.5 535,233 - +0.6 535,233 - -0.0 535,252 + +0.4 535,532 + BPHYT_RS02335 0.27 +0.0 535,532 + BPHYT_RS02335 0.27 +0.2 535,532 + BPHYT_RS02335 0.27 -1.1 535,554 + BPHYT_RS02335 0.29 -0.4 535,653 + BPHYT_RS02335 0.39 +1.2 535,653 + BPHYT_RS02335 0.39 -0.2 535,653 + BPHYT_RS02335 0.39 -0.9 535,653 + BPHYT_RS02335 0.39 -0.3 535,654 - BPHYT_RS02335 0.39 +0.6 535,654 - BPHYT_RS02335 0.39 +0.2 535,654 - BPHYT_RS02335 0.39 -0.7 536,094 - BPHYT_RS02335 0.84 +0.2 536,173 + -0.0 536,174 - -0.4 536,297 + +0.4 536,361 - -0.6 536,475 - +0.3 536,635 + BPHYT_RS02340 0.10 -1.8 536,636 - BPHYT_RS02340 0.10 -0.5 536,636 - BPHYT_RS02340 0.10 -0.5 536,677 + BPHYT_RS02340 0.14 -0.0 536,678 - BPHYT_RS02340 0.14 +0.3 536,773 - BPHYT_RS02340 0.21 -0.6 536,801 + BPHYT_RS02340 0.24 -0.8 536,802 - BPHYT_RS02340 0.24 -0.6 536,903 - BPHYT_RS02340 0.32 +1.5 537,105 + BPHYT_RS02340 0.48 +0.5 537,105 + BPHYT_RS02340 0.48 -1.8 537,203 + BPHYT_RS02340 0.56 -1.0 537,203 + BPHYT_RS02340 0.56 -0.5
Or see this region's nucleotide sequence