Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35330 and BPHYT_RS35335 are separated by 89 nucleotides BPHYT_RS35335 and BPHYT_RS35340 are separated by 40 nucleotides BPHYT_RS35340 and BPHYT_RS35345 overlap by 4 nucleotides
BPHYT_RS35330: BPHYT_RS35330 - purine permease, at 3,525,282 to 3,526,622
_RS35330
BPHYT_RS35335: BPHYT_RS35335 - cobalamin biosynthesis protein, at 3,526,712 to 3,527,794
_RS35335
BPHYT_RS35340: BPHYT_RS35340 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 3,527,835 to 3,528,425
_RS35340
BPHYT_RS35345: BPHYT_RS35345 - decarboxylase UbiD, at 3,528,422 to 3,529,816
_RS35345
Position (kb) 3526
3527
3528 Strain fitness (log2 ratio) -1
0
1 at 3526.639 kb on - strand at 3527.187 kb on + strand, within BPHYT_RS35335 at 3527.187 kb on + strand, within BPHYT_RS35335 at 3527.291 kb on - strand, within BPHYT_RS35335 at 3527.697 kb on + strand at 3527.833 kb on + strand at 3527.845 kb on + strand at 3527.846 kb on - strand at 3528.015 kb on + strand, within BPHYT_RS35340 at 3528.066 kb on + strand, within BPHYT_RS35340 at 3528.236 kb on - strand, within BPHYT_RS35340 at 3528.361 kb on - strand, within BPHYT_RS35340 at 3528.604 kb on + strand, within BPHYT_RS35345
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,526,639 - +1.0 3,527,187 + BPHYT_RS35335 0.44 +0.6 3,527,187 + BPHYT_RS35335 0.44 +0.2 3,527,291 - BPHYT_RS35335 0.53 +0.5 3,527,697 + +0.0 3,527,833 + -0.9 3,527,845 + +0.2 3,527,846 - +1.3 3,528,015 + BPHYT_RS35340 0.30 -1.0 3,528,066 + BPHYT_RS35340 0.39 -0.6 3,528,236 - BPHYT_RS35340 0.68 -0.2 3,528,361 - BPHYT_RS35340 0.89 +0.9 3,528,604 + BPHYT_RS35345 0.13 +0.9
Or see this region's nucleotide sequence