Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34655 and BPHYT_RS34660 are separated by 149 nucleotides BPHYT_RS34660 and BPHYT_RS34665 are separated by 105 nucleotides BPHYT_RS34665 and BPHYT_RS34670 are separated by 62 nucleotides
BPHYT_RS34655: BPHYT_RS34655 - 2-amino-3-ketobutyrate CoA ligase, at 3,381,848 to 3,383,047
_RS34655
BPHYT_RS34660: BPHYT_RS34660 - DNA-binding protein, at 3,383,197 to 3,383,835
_RS34660
BPHYT_RS34665: BPHYT_RS34665 - hypothetical protein, at 3,383,941 to 3,384,336
_RS34665
BPHYT_RS34670: BPHYT_RS34670 - integrase, at 3,384,399 to 3,385,481
_RS34670
Position (kb)
3383
3384
3385 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3382.983 kb on + strand at 3383.035 kb on - strand at 3383.122 kb on - strand at 3383.122 kb on - strand at 3383.124 kb on - strand at 3383.124 kb on - strand at 3383.558 kb on - strand, within BPHYT_RS34660 at 3383.558 kb on - strand at 3383.642 kb on + strand, within BPHYT_RS34660 at 3383.642 kb on + strand, within BPHYT_RS34660 at 3383.643 kb on - strand, within BPHYT_RS34660 at 3383.646 kb on + strand, within BPHYT_RS34660 at 3383.646 kb on + strand, within BPHYT_RS34660 at 3383.647 kb on - strand, within BPHYT_RS34660 at 3383.755 kb on + strand, within BPHYT_RS34660 at 3383.816 kb on + strand at 3383.904 kb on - strand at 3383.906 kb on - strand at 3383.992 kb on + strand, within BPHYT_RS34665 at 3384.046 kb on + strand, within BPHYT_RS34665 at 3384.046 kb on + strand, within BPHYT_RS34665 at 3384.047 kb on - strand, within BPHYT_RS34665 at 3384.070 kb on - strand, within BPHYT_RS34665 at 3384.368 kb on - strand at 3384.382 kb on + strand at 3384.553 kb on - strand, within BPHYT_RS34670 at 3384.685 kb on + strand, within BPHYT_RS34670 at 3384.974 kb on + strand, within BPHYT_RS34670 at 3385.124 kb on - strand, within BPHYT_RS34670 at 3385.124 kb on - strand, within BPHYT_RS34670 at 3385.158 kb on - strand, within BPHYT_RS34670 at 3385.158 kb on - strand, within BPHYT_RS34670 at 3385.158 kb on - strand, within BPHYT_RS34670
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,382,983 + -0.0 3,383,035 - -1.0 3,383,122 - +2.5 3,383,122 - -0.1 3,383,124 - +0.9 3,383,124 - -0.7 3,383,558 - BPHYT_RS34660 0.56 -0.3 3,383,558 - +2.3 3,383,642 + BPHYT_RS34660 0.70 +0.7 3,383,642 + BPHYT_RS34660 0.70 +0.5 3,383,643 - BPHYT_RS34660 0.70 +0.6 3,383,646 + BPHYT_RS34660 0.70 -1.2 3,383,646 + BPHYT_RS34660 0.70 -1.7 3,383,647 - BPHYT_RS34660 0.70 -0.4 3,383,755 + BPHYT_RS34660 0.87 -2.1 3,383,816 + +0.7 3,383,904 - +2.4 3,383,906 - -0.3 3,383,992 + BPHYT_RS34665 0.13 -0.2 3,384,046 + BPHYT_RS34665 0.27 +1.1 3,384,046 + BPHYT_RS34665 0.27 +0.6 3,384,047 - BPHYT_RS34665 0.27 +0.0 3,384,070 - BPHYT_RS34665 0.33 +2.8 3,384,368 - -0.3 3,384,382 + -0.2 3,384,553 - BPHYT_RS34670 0.14 -0.1 3,384,685 + BPHYT_RS34670 0.26 +2.1 3,384,974 + BPHYT_RS34670 0.53 +0.3 3,385,124 - BPHYT_RS34670 0.67 +0.1 3,385,124 - BPHYT_RS34670 0.67 +1.7 3,385,158 - BPHYT_RS34670 0.70 -0.7 3,385,158 - BPHYT_RS34670 0.70 -0.1 3,385,158 - BPHYT_RS34670 0.70 -0.8
Or see this region's nucleotide sequence