Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34630 and BPHYT_RS34635 are separated by 212 nucleotides BPHYT_RS34635 and BPHYT_RS34640 are separated by 13 nucleotides BPHYT_RS34640 and BPHYT_RS34645 are separated by 108 nucleotides BPHYT_RS34645 and BPHYT_RS34650 are separated by 97 nucleotides
BPHYT_RS34630: BPHYT_RS34630 - hypothetical protein, at 3,378,057 to 3,378,575
_RS34630
BPHYT_RS34635: BPHYT_RS34635 - 50S ribosomal protein L21, at 3,378,788 to 3,379,267
_RS34635
BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946
_RS34640
BPHYT_RS34645: BPHYT_RS34645 - hypothetical protein, at 3,380,055 to 3,380,705
_RS34645
BPHYT_RS34650: BPHYT_RS34650 - l-threonine 3-dehydrogenase, at 3,380,803 to 3,381,834
_RS34650
Position (kb)
3379
3380 Strain fitness (log2 ratio)
-1
0
1 at 3378.308 kb on - strand, within BPHYT_RS34630 at 3378.311 kb on - strand, within BPHYT_RS34630 at 3378.439 kb on + strand, within BPHYT_RS34630 at 3378.611 kb on + strand at 3378.611 kb on + strand at 3378.612 kb on - strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.628 kb on + strand at 3378.629 kb on - strand at 3378.629 kb on - strand at 3378.629 kb on - strand at 3378.849 kb on + strand, within BPHYT_RS34635 at 3378.984 kb on + strand, within BPHYT_RS34635 at 3378.985 kb on - strand, within BPHYT_RS34635 at 3379.270 kb on + strand at 3379.271 kb on - strand at 3379.793 kb on - strand, within BPHYT_RS34640 at 3379.865 kb on - strand, within BPHYT_RS34640 at 3379.948 kb on + strand at 3379.949 kb on - strand at 3380.019 kb on + strand at 3380.020 kb on - strand at 3380.020 kb on - strand at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.148 kb on - strand, within BPHYT_RS34645 at 3380.154 kb on - strand, within BPHYT_RS34645 at 3380.247 kb on + strand, within BPHYT_RS34645 at 3380.248 kb on - strand, within BPHYT_RS34645 at 3380.635 kb on - strand, within BPHYT_RS34645 at 3380.654 kb on + strand at 3380.800 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,378,308 - BPHYT_RS34630 0.48 -1.1 3,378,311 - BPHYT_RS34630 0.49 -1.3 3,378,439 + BPHYT_RS34630 0.74 +0.2 3,378,611 + +0.7 3,378,611 + -0.2 3,378,612 - +0.3 3,378,628 + -0.5 3,378,628 + -0.4 3,378,628 + -1.3 3,378,628 + -0.5 3,378,629 - -0.1 3,378,629 - -1.0 3,378,629 - +0.1 3,378,849 + BPHYT_RS34635 0.13 +0.1 3,378,984 + BPHYT_RS34635 0.41 +0.1 3,378,985 - BPHYT_RS34635 0.41 -0.5 3,379,270 + +1.0 3,379,271 - -0.3 3,379,793 - BPHYT_RS34640 0.77 +1.7 3,379,865 - BPHYT_RS34640 0.88 +0.6 3,379,948 + +0.7 3,379,949 - +0.5 3,380,019 + -1.2 3,380,020 - -1.7 3,380,020 - +1.3 3,380,148 - BPHYT_RS34645 0.14 -0.4 3,380,148 - BPHYT_RS34645 0.14 +0.3 3,380,154 - BPHYT_RS34645 0.15 +0.5 3,380,247 + BPHYT_RS34645 0.29 +1.3 3,380,248 - BPHYT_RS34645 0.30 -1.1 3,380,635 - BPHYT_RS34645 0.89 -0.3 3,380,654 + +0.0 3,380,800 - +0.5
Or see this region's nucleotide sequence