Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34640

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34630 and BPHYT_RS34635 are separated by 212 nucleotidesBPHYT_RS34635 and BPHYT_RS34640 are separated by 13 nucleotidesBPHYT_RS34640 and BPHYT_RS34645 are separated by 108 nucleotidesBPHYT_RS34645 and BPHYT_RS34650 are separated by 97 nucleotides BPHYT_RS34630: BPHYT_RS34630 - hypothetical protein, at 3,378,057 to 3,378,575 _RS34630 BPHYT_RS34635: BPHYT_RS34635 - 50S ribosomal protein L21, at 3,378,788 to 3,379,267 _RS34635 BPHYT_RS34640: BPHYT_RS34640 - TetR family transcriptional regulator, at 3,379,281 to 3,379,946 _RS34640 BPHYT_RS34645: BPHYT_RS34645 - hypothetical protein, at 3,380,055 to 3,380,705 _RS34645 BPHYT_RS34650: BPHYT_RS34650 - l-threonine 3-dehydrogenase, at 3,380,803 to 3,381,834 _RS34650 Position (kb) 3379 3380Strain fitness (log2 ratio) -1 0 1at 3378.308 kb on - strand, within BPHYT_RS34630at 3378.311 kb on - strand, within BPHYT_RS34630at 3378.439 kb on + strand, within BPHYT_RS34630at 3378.611 kb on + strandat 3378.611 kb on + strandat 3378.612 kb on - strandat 3378.628 kb on + strandat 3378.628 kb on + strandat 3378.628 kb on + strandat 3378.628 kb on + strandat 3378.629 kb on - strandat 3378.629 kb on - strandat 3378.629 kb on - strandat 3378.849 kb on + strand, within BPHYT_RS34635at 3378.984 kb on + strand, within BPHYT_RS34635at 3378.985 kb on - strand, within BPHYT_RS34635at 3379.270 kb on + strandat 3379.271 kb on - strandat 3379.793 kb on - strand, within BPHYT_RS34640at 3379.865 kb on - strand, within BPHYT_RS34640at 3379.948 kb on + strandat 3379.949 kb on - strandat 3380.019 kb on + strandat 3380.020 kb on - strandat 3380.020 kb on - strandat 3380.148 kb on - strand, within BPHYT_RS34645at 3380.148 kb on - strand, within BPHYT_RS34645at 3380.154 kb on - strand, within BPHYT_RS34645at 3380.247 kb on + strand, within BPHYT_RS34645at 3380.248 kb on - strand, within BPHYT_RS34645at 3380.635 kb on - strand, within BPHYT_RS34645at 3380.654 kb on + strandat 3380.800 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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3,378,308 - BPHYT_RS34630 0.48 -1.1
3,378,311 - BPHYT_RS34630 0.49 -1.3
3,378,439 + BPHYT_RS34630 0.74 +0.2
3,378,611 + +0.7
3,378,611 + -0.2
3,378,612 - +0.3
3,378,628 + -0.5
3,378,628 + -0.4
3,378,628 + -1.3
3,378,628 + -0.5
3,378,629 - -0.1
3,378,629 - -1.0
3,378,629 - +0.1
3,378,849 + BPHYT_RS34635 0.13 +0.1
3,378,984 + BPHYT_RS34635 0.41 +0.1
3,378,985 - BPHYT_RS34635 0.41 -0.5
3,379,270 + +1.0
3,379,271 - -0.3
3,379,793 - BPHYT_RS34640 0.77 +1.7
3,379,865 - BPHYT_RS34640 0.88 +0.6
3,379,948 + +0.7
3,379,949 - +0.5
3,380,019 + -1.2
3,380,020 - -1.7
3,380,020 - +1.3
3,380,148 - BPHYT_RS34645 0.14 -0.4
3,380,148 - BPHYT_RS34645 0.14 +0.3
3,380,154 - BPHYT_RS34645 0.15 +0.5
3,380,247 + BPHYT_RS34645 0.29 +1.3
3,380,248 - BPHYT_RS34645 0.30 -1.1
3,380,635 - BPHYT_RS34645 0.89 -0.3
3,380,654 + +0.0
3,380,800 - +0.5

Or see this region's nucleotide sequence