Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33220 and BPHYT_RS33225 are separated by 472 nucleotides
BPHYT_RS33220: BPHYT_RS33220 - group 1 glycosyl transferase, at 3,030,646 to 3,032,643
_RS33220
BPHYT_RS33225: BPHYT_RS33225 - NAD-dependent epimerase/dehydratase, at 3,033,116 to 3,034,060
_RS33225
Position (kb)
3033
3034
3035 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3032.117 kb on + strand, within BPHYT_RS33220 at 3032.129 kb on + strand, within BPHYT_RS33220 at 3032.130 kb on - strand, within BPHYT_RS33220 at 3032.133 kb on - strand, within BPHYT_RS33220 at 3032.133 kb on - strand, within BPHYT_RS33220 at 3032.269 kb on - strand, within BPHYT_RS33220 at 3032.628 kb on + strand at 3032.664 kb on + strand at 3032.720 kb on - strand at 3033.075 kb on + strand at 3033.151 kb on - strand at 3033.151 kb on - strand at 3033.332 kb on + strand, within BPHYT_RS33225 at 3033.575 kb on - strand, within BPHYT_RS33225 at 3033.606 kb on + strand, within BPHYT_RS33225 at 3033.614 kb on + strand, within BPHYT_RS33225 at 3033.619 kb on - strand, within BPHYT_RS33225 at 3033.656 kb on + strand, within BPHYT_RS33225 at 3033.657 kb on - strand, within BPHYT_RS33225 at 3033.773 kb on + strand, within BPHYT_RS33225 at 3033.796 kb on + strand, within BPHYT_RS33225 at 3034.065 kb on + strand at 3034.111 kb on - strand at 3034.127 kb on - strand at 3034.194 kb on - strand at 3034.225 kb on - strand at 3034.225 kb on - strand at 3034.258 kb on - strand at 3034.397 kb on - strand at 3034.409 kb on + strand at 3034.457 kb on - strand at 3034.463 kb on + strand at 3034.695 kb on - strand at 3034.756 kb on - strand at 3034.828 kb on - strand at 3034.877 kb on + strand at 3034.878 kb on - strand at 3034.997 kb on + strand at 3034.997 kb on + strand at 3034.998 kb on - strand at 3034.998 kb on - strand at 3034.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,032,117 + BPHYT_RS33220 0.74 -1.2 3,032,129 + BPHYT_RS33220 0.74 +1.6 3,032,130 - BPHYT_RS33220 0.74 -1.8 3,032,133 - BPHYT_RS33220 0.74 -0.9 3,032,133 - BPHYT_RS33220 0.74 -0.7 3,032,269 - BPHYT_RS33220 0.81 -1.5 3,032,628 + +0.7 3,032,664 + +2.3 3,032,720 - +1.4 3,033,075 + -0.5 3,033,151 - -0.7 3,033,151 - +1.3 3,033,332 + BPHYT_RS33225 0.23 -2.1 3,033,575 - BPHYT_RS33225 0.49 -0.9 3,033,606 + BPHYT_RS33225 0.52 -2.3 3,033,614 + BPHYT_RS33225 0.53 -2.4 3,033,619 - BPHYT_RS33225 0.53 -1.5 3,033,656 + BPHYT_RS33225 0.57 +0.7 3,033,657 - BPHYT_RS33225 0.57 -1.2 3,033,773 + BPHYT_RS33225 0.70 -2.7 3,033,796 + BPHYT_RS33225 0.72 +0.3 3,034,065 + -4.6 3,034,111 - +0.3 3,034,127 - +0.7 3,034,194 - +1.5 3,034,225 - +1.5 3,034,225 - +0.3 3,034,258 - -1.0 3,034,397 - +1.3 3,034,409 + +1.7 3,034,457 - -0.4 3,034,463 + -0.7 3,034,695 - -0.1 3,034,756 - +1.4 3,034,828 - +0.7 3,034,877 + -0.7 3,034,878 - +0.6 3,034,997 + +0.4 3,034,997 + +0.8 3,034,998 - +0.6 3,034,998 - +0.5 3,034,998 - +0.4
Or see this region's nucleotide sequence