Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30075 and BPHYT_RS30080 are separated by 204 nucleotides BPHYT_RS30080 and BPHYT_RS30085 are separated by 159 nucleotides BPHYT_RS30085 and BPHYT_RS30090 are separated by 124 nucleotides
BPHYT_RS30075: BPHYT_RS30075 - NADPH:quinone oxidoreductase, at 2,302,101 to 2,303,108
_RS30075
BPHYT_RS30080: BPHYT_RS30080 - antibiotic biosynthesis monooxygenase, at 2,303,313 to 2,303,627
_RS30080
BPHYT_RS30085: BPHYT_RS30085 - hypothetical protein, at 2,303,787 to 2,304,746
_RS30085
BPHYT_RS30090: BPHYT_RS30090 - hypothetical protein, at 2,304,871 to 2,306,454
_RS30090
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-1
0
1
2 at 2302.857 kb on + strand, within BPHYT_RS30075 at 2302.858 kb on - strand, within BPHYT_RS30075 at 2303.549 kb on + strand, within BPHYT_RS30080 at 2303.717 kb on + strand at 2303.758 kb on - strand at 2303.899 kb on - strand, within BPHYT_RS30085 at 2303.899 kb on - strand, within BPHYT_RS30085 at 2304.793 kb on - strand at 2305.002 kb on - strand at 2305.606 kb on + strand, within BPHYT_RS30090 at 2305.606 kb on + strand, within BPHYT_RS30090 at 2305.607 kb on - strand, within BPHYT_RS30090 at 2305.608 kb on + strand, within BPHYT_RS30090 at 2305.609 kb on - strand, within BPHYT_RS30090
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,302,857 + BPHYT_RS30075 0.75 +1.4 2,302,858 - BPHYT_RS30075 0.75 -0.7 2,303,549 + BPHYT_RS30080 0.75 +1.1 2,303,717 + -0.1 2,303,758 - +0.3 2,303,899 - BPHYT_RS30085 0.12 -0.0 2,303,899 - BPHYT_RS30085 0.12 +1.5 2,304,793 - +0.7 2,305,002 - -0.8 2,305,606 + BPHYT_RS30090 0.46 -1.2 2,305,606 + BPHYT_RS30090 0.46 +2.1 2,305,607 - BPHYT_RS30090 0.46 -0.1 2,305,608 + BPHYT_RS30090 0.47 -1.6 2,305,609 - BPHYT_RS30090 0.47 +0.4
Or see this region's nucleotide sequence