Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29135 and BPHYT_RS29140 are separated by 323 nucleotides BPHYT_RS29140 and BPHYT_RS29145 are separated by 145 nucleotides BPHYT_RS29145 and BPHYT_RS29150 are separated by 164 nucleotides BPHYT_RS29150 and BPHYT_RS29155 are separated by 69 nucleotides
BPHYT_RS29135: BPHYT_RS29135 - acriflavin resistance protein, at 2,090,455 to 2,093,703
_RS29135
BPHYT_RS29140: BPHYT_RS29140 - glyoxalase, at 2,094,027 to 2,094,461
_RS29140
BPHYT_RS29145: BPHYT_RS29145 - LysR family transcriptional regulator, at 2,094,607 to 2,095,491
_RS29145
BPHYT_RS29150: BPHYT_RS29150 - lysine transporter LysE, at 2,095,656 to 2,096,294
_RS29150
BPHYT_RS29155: BPHYT_RS29155 - hypothetical protein, at 2,096,364 to 2,096,711
_RS29155
Position (kb)
2094
2095
2096 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2093.756 kb on + strand at 2094.009 kb on - strand at 2094.826 kb on + strand, within BPHYT_RS29145 at 2095.509 kb on + strand at 2095.543 kb on + strand at 2095.544 kb on - strand at 2095.544 kb on - strand at 2095.914 kb on + strand, within BPHYT_RS29150 at 2096.208 kb on + strand, within BPHYT_RS29150 at 2096.424 kb on + strand, within BPHYT_RS29155 at 2096.425 kb on - strand, within BPHYT_RS29155 at 2096.478 kb on + strand, within BPHYT_RS29155 at 2096.479 kb on - strand, within BPHYT_RS29155
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,093,756 + +1.3 2,094,009 - +1.9 2,094,826 + BPHYT_RS29145 0.25 -1.0 2,095,509 + -1.4 2,095,543 + -0.9 2,095,544 - -2.2 2,095,544 - -0.8 2,095,914 + BPHYT_RS29150 0.40 +0.4 2,096,208 + BPHYT_RS29150 0.86 +0.5 2,096,424 + BPHYT_RS29155 0.17 +0.9 2,096,425 - BPHYT_RS29155 0.18 -0.9 2,096,478 + BPHYT_RS29155 0.33 -1.5 2,096,479 - BPHYT_RS29155 0.33 +0.3
Or see this region's nucleotide sequence