Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29015 and BPHYT_RS29020 are separated by 113 nucleotides BPHYT_RS29020 and BPHYT_RS29025 are separated by 410 nucleotides BPHYT_RS29025 and BPHYT_RS29030 are separated by 377 nucleotides BPHYT_RS29030 and BPHYT_RS29035 are separated by 69 nucleotides
BPHYT_RS29015: BPHYT_RS29015 - RND transporter, at 2,066,861 to 2,068,432
_RS29015
BPHYT_RS29020: BPHYT_RS29020 - hypothetical protein, at 2,068,546 to 2,068,836
_RS29020
BPHYT_RS29025: BPHYT_RS29025 - hypothetical protein, at 2,069,247 to 2,069,582
_RS29025
BPHYT_RS29030: BPHYT_RS29030 - hypothetical protein, at 2,069,960 to 2,070,205
_RS29030
BPHYT_RS29035: BPHYT_RS29035 - arsenic transporter, at 2,070,275 to 2,071,525
_RS29035
Position (kb)
2069
2070 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2068.601 kb on + strand, within BPHYT_RS29020 at 2069.404 kb on - strand, within BPHYT_RS29025 at 2069.642 kb on + strand at 2069.642 kb on + strand at 2069.695 kb on - strand at 2069.753 kb on - strand at 2069.967 kb on - strand at 2070.214 kb on + strand at 2070.225 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,068,601 + BPHYT_RS29020 0.19 -1.2 2,069,404 - BPHYT_RS29025 0.47 +0.2 2,069,642 + -0.1 2,069,642 + -1.2 2,069,695 - +0.3 2,069,753 - +0.3 2,069,967 - -2.9 2,070,214 + +0.0 2,070,225 + +0.1
Or see this region's nucleotide sequence