Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28645 and BPHYT_RS28650 overlap by 16 nucleotides BPHYT_RS28650 and BPHYT_RS28655 overlap by 8 nucleotides BPHYT_RS28655 and BPHYT_RS28660 are separated by 1 nucleotides BPHYT_RS28660 and BPHYT_RS28665 are separated by 782 nucleotides
BPHYT_RS28645: BPHYT_RS28645 - serine/threonine protein kinase, at 1,981,049 to 1,982,041
_RS28645
BPHYT_RS28650: BPHYT_RS28650 - anti-sigma regulatory factor, at 1,982,026 to 1,982,436
_RS28650
BPHYT_RS28655: BPHYT_RS28655 - anti-sigma factor antagonist, at 1,982,429 to 1,982,806
_RS28655
BPHYT_RS28660: BPHYT_RS28660 - polyvinylalcohol dehydrogenase, at 1,982,808 to 1,983,674
_RS28660
BPHYT_RS28665: BPHYT_RS28665 - IclR family transcriptional regulator, at 1,984,457 to 1,985,272
_RS28665
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1981.844 kb on - strand, within BPHYT_RS28645 at 1981.844 kb on - strand, within BPHYT_RS28645 at 1981.858 kb on - strand, within BPHYT_RS28645 at 1981.890 kb on + strand, within BPHYT_RS28645 at 1981.891 kb on - strand, within BPHYT_RS28645 at 1981.898 kb on + strand, within BPHYT_RS28645 at 1981.900 kb on + strand, within BPHYT_RS28645 at 1982.046 kb on + strand at 1982.338 kb on - strand, within BPHYT_RS28650 at 1982.806 kb on + strand at 1983.253 kb on + strand, within BPHYT_RS28660 at 1983.552 kb on + strand, within BPHYT_RS28660 at 1983.618 kb on - strand at 1983.618 kb on - strand at 1983.766 kb on - strand at 1983.768 kb on - strand at 1983.928 kb on + strand at 1983.928 kb on + strand at 1983.989 kb on - strand at 1984.149 kb on - strand at 1984.152 kb on + strand at 1984.152 kb on + strand at 1984.156 kb on + strand at 1984.335 kb on + strand at 1984.467 kb on + strand at 1984.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,981,844 - BPHYT_RS28645 0.80 +1.8 1,981,844 - BPHYT_RS28645 0.80 +0.7 1,981,858 - BPHYT_RS28645 0.81 +0.2 1,981,890 + BPHYT_RS28645 0.85 -2.0 1,981,891 - BPHYT_RS28645 0.85 +0.8 1,981,898 + BPHYT_RS28645 0.85 +0.3 1,981,900 + BPHYT_RS28645 0.86 -0.6 1,982,046 + -1.3 1,982,338 - BPHYT_RS28650 0.76 +0.5 1,982,806 + +0.0 1,983,253 + BPHYT_RS28660 0.51 -0.6 1,983,552 + BPHYT_RS28660 0.86 -2.3 1,983,618 - +0.1 1,983,618 - +0.8 1,983,766 - -1.3 1,983,768 - -0.4 1,983,928 + -0.6 1,983,928 + -0.3 1,983,989 - -1.2 1,984,149 - +0.9 1,984,152 + -0.8 1,984,152 + -1.9 1,984,156 + +0.3 1,984,335 + +0.8 1,984,467 + +0.3 1,984,468 - +1.0
Or see this region's nucleotide sequence