Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28130 and BPHYT_RS28135 are separated by 74 nucleotides BPHYT_RS28135 and BPHYT_RS28140 are separated by 337 nucleotides BPHYT_RS28140 and BPHYT_RS28145 are separated by 57 nucleotides
BPHYT_RS28130: BPHYT_RS28130 - GntR family transcriptional regulator, at 1,869,357 to 1,870,100
_RS28130
BPHYT_RS28135: BPHYT_RS28135 - alcohol dehydrogenase, at 1,870,175 to 1,871,194
_RS28135
BPHYT_RS28140: BPHYT_RS28140 - translation initiation factor SUI1, at 1,871,532 to 1,871,894
_RS28140
BPHYT_RS28145: BPHYT_RS28145 - diguanylate cyclase, at 1,871,952 to 1,873,133
_RS28145
Position (kb)
1870
1871
1872 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1869.344 kb on + strand at 1869.348 kb on + strand at 1869.580 kb on - strand, within BPHYT_RS28130 at 1869.729 kb on - strand, within BPHYT_RS28130 at 1870.003 kb on + strand, within BPHYT_RS28130 at 1870.004 kb on - strand, within BPHYT_RS28130 at 1870.110 kb on - strand at 1870.830 kb on + strand, within BPHYT_RS28135 at 1870.843 kb on - strand, within BPHYT_RS28135 at 1870.843 kb on - strand, within BPHYT_RS28135 at 1870.985 kb on - strand, within BPHYT_RS28135 at 1871.362 kb on + strand at 1871.387 kb on + strand at 1871.388 kb on - strand at 1871.388 kb on - strand at 1871.445 kb on - strand at 1871.670 kb on + strand, within BPHYT_RS28140 at 1871.917 kb on + strand at 1871.917 kb on + strand at 1871.918 kb on - strand at 1871.918 kb on - strand at 1872.117 kb on + strand, within BPHYT_RS28145 at 1872.118 kb on - strand, within BPHYT_RS28145
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,869,344 + +0.7 1,869,348 + +1.9 1,869,580 - BPHYT_RS28130 0.30 -0.4 1,869,729 - BPHYT_RS28130 0.50 +0.8 1,870,003 + BPHYT_RS28130 0.87 -1.1 1,870,004 - BPHYT_RS28130 0.87 -1.0 1,870,110 - -1.5 1,870,830 + BPHYT_RS28135 0.64 -1.5 1,870,843 - BPHYT_RS28135 0.65 +0.0 1,870,843 - BPHYT_RS28135 0.65 -1.0 1,870,985 - BPHYT_RS28135 0.79 -1.5 1,871,362 + -0.3 1,871,387 + +1.3 1,871,388 - +1.3 1,871,388 - +0.3 1,871,445 - -1.2 1,871,670 + BPHYT_RS28140 0.38 -0.6 1,871,917 + -0.1 1,871,917 + +1.2 1,871,918 - +1.3 1,871,918 - -0.6 1,872,117 + BPHYT_RS28145 0.14 -2.8 1,872,118 - BPHYT_RS28145 0.14 +0.3
Or see this region's nucleotide sequence