Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28120 and BPHYT_RS28125 are separated by 131 nucleotides BPHYT_RS28125 and BPHYT_RS28130 are separated by 299 nucleotides BPHYT_RS28130 and BPHYT_RS28135 are separated by 74 nucleotides
BPHYT_RS28120: BPHYT_RS28120 - hypothetical protein, at 1,868,372 to 1,868,653
_RS28120
BPHYT_RS28125: BPHYT_RS28125 - Translation initiation factor IF-1 2, at 1,868,785 to 1,869,057
_RS28125
BPHYT_RS28130: BPHYT_RS28130 - GntR family transcriptional regulator, at 1,869,357 to 1,870,100
_RS28130
BPHYT_RS28135: BPHYT_RS28135 - alcohol dehydrogenase, at 1,870,175 to 1,871,194
_RS28135
Position (kb)
1869
1870
1871 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1868.393 kb on + strand at 1868.393 kb on + strand at 1868.394 kb on - strand at 1868.671 kb on + strand at 1868.671 kb on + strand at 1868.672 kb on - strand at 1868.680 kb on - strand at 1868.762 kb on - strand at 1868.780 kb on + strand at 1868.780 kb on + strand at 1868.780 kb on + strand at 1868.780 kb on + strand at 1868.780 kb on + strand at 1868.780 kb on + strand at 1868.781 kb on - strand at 1868.781 kb on - strand at 1869.344 kb on + strand at 1869.348 kb on + strand at 1869.580 kb on - strand, within BPHYT_RS28130 at 1869.729 kb on - strand, within BPHYT_RS28130 at 1870.003 kb on + strand, within BPHYT_RS28130 at 1870.004 kb on - strand, within BPHYT_RS28130 at 1870.110 kb on - strand at 1870.830 kb on + strand, within BPHYT_RS28135 at 1870.843 kb on - strand, within BPHYT_RS28135 at 1870.843 kb on - strand, within BPHYT_RS28135 at 1870.985 kb on - strand, within BPHYT_RS28135
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,868,393 + +0.2 1,868,393 + -0.9 1,868,394 - +0.6 1,868,671 + +0.9 1,868,671 + +0.6 1,868,672 - -0.2 1,868,680 - -1.7 1,868,762 - +3.3 1,868,780 + +2.3 1,868,780 + -1.2 1,868,780 + +0.2 1,868,780 + -0.5 1,868,780 + +1.6 1,868,780 + -1.0 1,868,781 - +0.5 1,868,781 - -0.6 1,869,344 + +0.7 1,869,348 + +1.9 1,869,580 - BPHYT_RS28130 0.30 -0.4 1,869,729 - BPHYT_RS28130 0.50 +0.8 1,870,003 + BPHYT_RS28130 0.87 -1.1 1,870,004 - BPHYT_RS28130 0.87 -1.0 1,870,110 - -1.5 1,870,830 + BPHYT_RS28135 0.64 -1.5 1,870,843 - BPHYT_RS28135 0.65 +0.0 1,870,843 - BPHYT_RS28135 0.65 -1.0 1,870,985 - BPHYT_RS28135 0.79 -1.5
Or see this region's nucleotide sequence