Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27850

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27845 and BPHYT_RS27850 are separated by 58 nucleotidesBPHYT_RS27850 and BPHYT_RS27855 are separated by 622 nucleotides BPHYT_RS27845: BPHYT_RS27845 - beta-lactamase, at 1,809,020 to 1,811,341 _RS27845 BPHYT_RS27850: BPHYT_RS27850 - hemerythrin, at 1,811,400 to 1,811,972 _RS27850 BPHYT_RS27855: BPHYT_RS27855 - hypothetical protein, at 1,812,595 to 1,812,813 _RS27855 Position (kb) 1811 1812Strain fitness (log2 ratio) -2 -1 0 1 2at 1811.822 kb on - strand, within BPHYT_RS27850at 1812.156 kb on + strandat 1812.622 kb on + strand, within BPHYT_RS27855at 1812.622 kb on + strand, within BPHYT_RS27855at 1812.706 kb on - strand, within BPHYT_RS27855at 1812.819 kb on + strandat 1812.847 kb on + strandat 1812.865 kb on - strandat 1812.909 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,811,822 - BPHYT_RS27850 0.74 -2.4
1,812,156 + -0.7
1,812,622 + BPHYT_RS27855 0.12 +0.2
1,812,622 + BPHYT_RS27855 0.12 -0.7
1,812,706 - BPHYT_RS27855 0.51 +2.0
1,812,819 + +0.2
1,812,847 + +0.4
1,812,865 - -1.0
1,812,909 + +0.4

Or see this region's nucleotide sequence