Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26490 and BPHYT_RS26495 are separated by 36 nucleotides BPHYT_RS26495 and BPHYT_RS26500 are separated by 39 nucleotides BPHYT_RS26500 and BPHYT_RS26505 overlap by 4 nucleotides BPHYT_RS26505 and BPHYT_RS26510 are separated by 160 nucleotides
BPHYT_RS26490: BPHYT_RS26490 - hypothetical protein, at 1,514,795 to 1,515,007
_RS26490
BPHYT_RS26495: BPHYT_RS26495 - glutathione S-transferase, at 1,515,044 to 1,515,682
_RS26495
BPHYT_RS26500: BPHYT_RS26500 - ATPase, at 1,515,722 to 1,516,171
_RS26500
BPHYT_RS26505: BPHYT_RS26505 - ArsR family transcriptional regulator, at 1,516,168 to 1,516,503
_RS26505
BPHYT_RS26510: BPHYT_RS26510 - transcriptional regulator, at 1,516,664 to 1,517,569
_RS26510
Position (kb) 1515
1516
1517 Strain fitness (log2 ratio) -2
-1
0
1 at 1514.728 kb on + strand at 1514.729 kb on - strand at 1514.770 kb on - strand at 1514.775 kb on + strand at 1514.825 kb on - strand, within BPHYT_RS26490 at 1515.670 kb on - strand at 1516.857 kb on - strand, within BPHYT_RS26510 at 1516.865 kb on + strand, within BPHYT_RS26510
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,514,728 + -0.8 1,514,729 - +0.5 1,514,770 - -1.8 1,514,775 + +0.5 1,514,825 - BPHYT_RS26490 0.14 -2.1 1,515,670 - +0.1 1,516,857 - BPHYT_RS26510 0.21 -1.5 1,516,865 + BPHYT_RS26510 0.22 -0.2
Or see this region's nucleotide sequence