Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26380 and BPHYT_RS26385 are separated by 27 nucleotides BPHYT_RS26385 and BPHYT_RS26390 are separated by 87 nucleotides BPHYT_RS26390 and BPHYT_RS26395 are separated by 373 nucleotides BPHYT_RS26395 and BPHYT_RS26400 overlap by 4 nucleotides
BPHYT_RS26380: BPHYT_RS26380 - cobalamin biosynthesis protein CobT, at 1,491,243 to 1,492,991
_RS26380
BPHYT_RS26385: BPHYT_RS26385 - glutamine amidotransferase, at 1,493,019 to 1,493,852
_RS26385
BPHYT_RS26390: BPHYT_RS26390 - antibiotic resistance protein, at 1,493,940 to 1,494,617
_RS26390
BPHYT_RS26395: BPHYT_RS26395 - membrane protein, at 1,494,991 to 1,495,206
_RS26395
BPHYT_RS26400: BPHYT_RS26400 - sodium:solute symporter, at 1,495,203 to 1,496,675
_RS26400
Position (kb) 1493
1494
1495 Strain fitness (log2 ratio) -1
0
1
2 at 1493.498 kb on - strand, within BPHYT_RS26385 at 1493.853 kb on - strand at 1493.853 kb on - strand at 1494.182 kb on + strand, within BPHYT_RS26390 at 1494.617 kb on + strand at 1494.777 kb on + strand at 1494.857 kb on - strand at 1494.928 kb on + strand at 1494.964 kb on + strand at 1494.964 kb on + strand at 1495.450 kb on - strand, within BPHYT_RS26400
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,493,498 - BPHYT_RS26385 0.57 -1.2 1,493,853 - +0.0 1,493,853 - +1.5 1,494,182 + BPHYT_RS26390 0.36 +1.4 1,494,617 + -0.3 1,494,777 + -0.5 1,494,857 - +0.2 1,494,928 + -0.2 1,494,964 + +2.0 1,494,964 + +0.7 1,495,450 - BPHYT_RS26400 0.17 -1.2
Or see this region's nucleotide sequence