Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25585

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25575 and BPHYT_RS25580 are separated by 93 nucleotidesBPHYT_RS25580 and BPHYT_RS25585 are separated by 258 nucleotidesBPHYT_RS25585 and BPHYT_RS25590 are separated by 623 nucleotides BPHYT_RS25575: BPHYT_RS25575 - alpha/beta hydrolase, at 1,309,885 to 1,310,751 _RS25575 BPHYT_RS25580: BPHYT_RS25580 - membrane protein, at 1,310,845 to 1,311,318 _RS25580 BPHYT_RS25585: BPHYT_RS25585 - membrane protein, at 1,311,577 to 1,311,888 _RS25585 BPHYT_RS25590: BPHYT_RS25590 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 1,312,512 to 1,314,602 _RS25590 Position (kb) 1311 1312Strain fitness (log2 ratio) -1 0 1at 1310.876 kb on + strandat 1311.335 kb on + strandat 1311.338 kb on - strandat 1311.801 kb on + strand, within BPHYT_RS25585at 1311.955 kb on - strandat 1312.571 kb on + strandat 1312.572 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,310,876 + +0.4
1,311,335 + -0.8
1,311,338 - +0.4
1,311,801 + BPHYT_RS25585 0.72 -1.6
1,311,955 - +0.1
1,312,571 + -0.4
1,312,572 - -1.2

Or see this region's nucleotide sequence