Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25565

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25555 and BPHYT_RS25560 are separated by 100 nucleotidesBPHYT_RS25560 and BPHYT_RS25565 overlap by 4 nucleotidesBPHYT_RS25565 and BPHYT_RS25570 are separated by 272 nucleotides BPHYT_RS25555: BPHYT_RS25555 - alkyl hydroperoxide reductase, at 1,306,880 to 1,307,560 _RS25555 BPHYT_RS25560: BPHYT_RS25560 - RNA polymerase sigma factor, at 1,307,661 to 1,308,233 _RS25560 BPHYT_RS25565: BPHYT_RS25565 - hypothetical protein, at 1,308,230 to 1,308,871 _RS25565 BPHYT_RS25570: BPHYT_RS25570 - hypothetical protein, at 1,309,144 to 1,309,389 _RS25570 Position (kb) 1308 1309Strain fitness (log2 ratio) -2 -1 0 1at 1307.935 kb on - strand, within BPHYT_RS25560at 1307.936 kb on + strand, within BPHYT_RS25560at 1308.616 kb on - strand, within BPHYT_RS25565at 1308.626 kb on - strand, within BPHYT_RS25565at 1308.862 kb on - strandat 1308.964 kb on - strandat 1309.536 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,307,935 - BPHYT_RS25560 0.48 -0.1
1,307,936 + BPHYT_RS25560 0.48 -0.8
1,308,616 - BPHYT_RS25565 0.60 -0.8
1,308,626 - BPHYT_RS25565 0.62 +1.0
1,308,862 - +0.4
1,308,964 - +0.5
1,309,536 - -2.2

Or see this region's nucleotide sequence