Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25130

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25120 and BPHYT_RS25125 are separated by 57 nucleotidesBPHYT_RS25125 and BPHYT_RS25130 are separated by 142 nucleotidesBPHYT_RS25130 and BPHYT_RS25135 are separated by 3 nucleotidesBPHYT_RS25135 and BPHYT_RS25140 overlap by 4 nucleotides BPHYT_RS25120: BPHYT_RS25120 - sarcosine oxidase subunit gamma, at 1,209,673 to 1,210,329 _RS25120 BPHYT_RS25125: BPHYT_RS25125 - hypothetical protein, at 1,210,387 to 1,210,650 _RS25125 BPHYT_RS25130: BPHYT_RS25130 - MFS transporter, at 1,210,793 to 1,212,211 _RS25130 BPHYT_RS25135: BPHYT_RS25135 - hypothetical protein, at 1,212,215 to 1,212,658 _RS25135 BPHYT_RS25140: BPHYT_RS25140 - hypothetical protein, at 1,212,655 to 1,213,164 _RS25140 Position (kb) 1210 1211 1212 1213Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1210.282 kb on + strandat 1210.711 kb on + strandat 1210.712 kb on - strandat 1211.284 kb on + strand, within BPHYT_RS25130at 1211.364 kb on + strand, within BPHYT_RS25130at 1211.822 kb on + strand, within BPHYT_RS25130at 1211.826 kb on + strand, within BPHYT_RS25130at 1212.020 kb on + strand, within BPHYT_RS25130at 1212.021 kb on - strand, within BPHYT_RS25130at 1212.216 kb on + strandat 1212.519 kb on + strand, within BPHYT_RS25135at 1212.629 kb on - strandat 1213.031 kb on - strand, within BPHYT_RS25140at 1213.082 kb on + strand, within BPHYT_RS25140at 1213.193 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,210,282 + +0.2
1,210,711 + -2.1
1,210,712 - +0.1
1,211,284 + BPHYT_RS25130 0.35 +1.2
1,211,364 + BPHYT_RS25130 0.40 -1.4
1,211,822 + BPHYT_RS25130 0.73 -2.0
1,211,826 + BPHYT_RS25130 0.73 +0.9
1,212,020 + BPHYT_RS25130 0.86 +0.2
1,212,021 - BPHYT_RS25130 0.87 +1.4
1,212,216 + +1.1
1,212,519 + BPHYT_RS25135 0.68 -1.3
1,212,629 - -1.5
1,213,031 - BPHYT_RS25140 0.74 -3.5
1,213,082 + BPHYT_RS25140 0.84 +3.2
1,213,193 + -1.7

Or see this region's nucleotide sequence