Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25030 and BPHYT_RS25035 are separated by 86 nucleotides BPHYT_RS25035 and BPHYT_RS25040 are separated by 197 nucleotides BPHYT_RS25040 and BPHYT_RS25045 are separated by 133 nucleotides
BPHYT_RS25030: BPHYT_RS25030 - gluconate 2-dehydrogenase, at 1,185,696 to 1,186,469
_RS25030
BPHYT_RS25035: BPHYT_RS25035 - long-chain fatty acid--CoA ligase, at 1,186,556 to 1,188,253
_RS25035
BPHYT_RS25040: BPHYT_RS25040 - hypothetical protein, at 1,188,451 to 1,188,741
_RS25040
BPHYT_RS25045: BPHYT_RS25045 - LysR family transcriptional regulator, at 1,188,875 to 1,189,786
_RS25045
Position (kb)
1186
1187
1188
1189 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1185.564 kb on - strand at 1185.633 kb on - strand at 1185.633 kb on - strand at 1185.965 kb on + strand, within BPHYT_RS25030 at 1186.186 kb on - strand, within BPHYT_RS25030 at 1186.344 kb on - strand, within BPHYT_RS25030 at 1186.486 kb on - strand at 1186.631 kb on + strand at 1186.792 kb on + strand, within BPHYT_RS25035 at 1186.823 kb on + strand, within BPHYT_RS25035 at 1187.015 kb on + strand, within BPHYT_RS25035 at 1187.015 kb on + strand, within BPHYT_RS25035 at 1187.413 kb on - strand, within BPHYT_RS25035 at 1187.499 kb on - strand, within BPHYT_RS25035 at 1187.592 kb on - strand, within BPHYT_RS25035 at 1188.330 kb on + strand at 1188.331 kb on - strand at 1188.960 kb on + strand at 1189.024 kb on - strand, within BPHYT_RS25045 at 1189.024 kb on - strand, within BPHYT_RS25045
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,185,564 - +0.3 1,185,633 - +0.5 1,185,633 - +0.8 1,185,965 + BPHYT_RS25030 0.35 +0.1 1,186,186 - BPHYT_RS25030 0.63 +1.7 1,186,344 - BPHYT_RS25030 0.84 -0.4 1,186,486 - +0.1 1,186,631 + +0.6 1,186,792 + BPHYT_RS25035 0.14 -2.0 1,186,823 + BPHYT_RS25035 0.16 -2.1 1,187,015 + BPHYT_RS25035 0.27 -2.8 1,187,015 + BPHYT_RS25035 0.27 -2.5 1,187,413 - BPHYT_RS25035 0.50 -0.7 1,187,499 - BPHYT_RS25035 0.56 -0.0 1,187,592 - BPHYT_RS25035 0.61 -0.2 1,188,330 + +0.7 1,188,331 - +0.7 1,188,960 + -0.3 1,189,024 - BPHYT_RS25045 0.16 -2.2 1,189,024 - BPHYT_RS25045 0.16 +0.2
Or see this region's nucleotide sequence