Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24210 and BPHYT_RS24215 are separated by 254 nucleotides BPHYT_RS24215 and BPHYT_RS24220 overlap by 26 nucleotides BPHYT_RS24220 and BPHYT_RS24225 overlap by 4 nucleotides BPHYT_RS24225 and BPHYT_RS24230 are separated by 82 nucleotides
BPHYT_RS24210: BPHYT_RS24210 - urea transporter, at 998,067 to 999,011
_RS24210
BPHYT_RS24215: BPHYT_RS24215 - transcriptional regulator, at 999,266 to 999,820
_RS24215
BPHYT_RS24220: BPHYT_RS24220 - alpha/beta hydrolase, at 999,795 to 1,000,760
_RS24220
BPHYT_RS24225: BPHYT_RS24225 - hypothetical protein, at 1,000,757 to 1,001,638
_RS24225
BPHYT_RS24230: BPHYT_RS24230 - acetoin dehydrogenase, at 1,001,721 to 1,002,629
_RS24230
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 998.816 kb on - strand, within BPHYT_RS24210 at 998.816 kb on - strand, within BPHYT_RS24210 at 999.103 kb on - strand at 999.387 kb on - strand, within BPHYT_RS24215 at 999.398 kb on + strand, within BPHYT_RS24215 at 999.399 kb on - strand, within BPHYT_RS24215 at 999.435 kb on - strand, within BPHYT_RS24215 at 999.641 kb on - strand, within BPHYT_RS24215 at 999.641 kb on - strand, within BPHYT_RS24215 at 999.642 kb on + strand, within BPHYT_RS24215 at 999.683 kb on + strand, within BPHYT_RS24215 at 999.683 kb on + strand, within BPHYT_RS24215 at 999.684 kb on - strand, within BPHYT_RS24215 at 999.998 kb on - strand, within BPHYT_RS24220 at 1000.585 kb on - strand, within BPHYT_RS24220 at 1000.708 kb on + strand at 1000.956 kb on + strand, within BPHYT_RS24225 at 1000.962 kb on + strand, within BPHYT_RS24225 at 1001.035 kb on - strand, within BPHYT_RS24225 at 1001.097 kb on + strand, within BPHYT_RS24225 at 1001.132 kb on + strand, within BPHYT_RS24225 at 1001.263 kb on - strand, within BPHYT_RS24225 at 1001.395 kb on - strand, within BPHYT_RS24225 at 1001.395 kb on - strand, within BPHYT_RS24225 at 1001.691 kb on - strand at 1001.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 998,816 - BPHYT_RS24210 0.79 +0.5 998,816 - BPHYT_RS24210 0.79 -0.8 999,103 - -0.1 999,387 - BPHYT_RS24215 0.22 +0.0 999,398 + BPHYT_RS24215 0.24 +0.1 999,399 - BPHYT_RS24215 0.24 +0.1 999,435 - BPHYT_RS24215 0.30 -0.5 999,641 - BPHYT_RS24215 0.68 +1.7 999,641 - BPHYT_RS24215 0.68 -0.1 999,642 + BPHYT_RS24215 0.68 -1.3 999,683 + BPHYT_RS24215 0.75 -0.6 999,683 + BPHYT_RS24215 0.75 +0.6 999,684 - BPHYT_RS24215 0.75 +0.3 999,998 - BPHYT_RS24220 0.21 -0.6 1,000,585 - BPHYT_RS24220 0.82 -1.6 1,000,708 + -1.1 1,000,956 + BPHYT_RS24225 0.23 -1.7 1,000,962 + BPHYT_RS24225 0.23 +0.1 1,001,035 - BPHYT_RS24225 0.32 +0.3 1,001,097 + BPHYT_RS24225 0.39 -2.9 1,001,132 + BPHYT_RS24225 0.43 +0.2 1,001,263 - BPHYT_RS24225 0.57 +0.0 1,001,395 - BPHYT_RS24225 0.72 -0.4 1,001,395 - BPHYT_RS24225 0.72 -0.9 1,001,691 - -1.5 1,001,691 - +0.2
Or see this region's nucleotide sequence