Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23735 and BPHYT_RS23740 are separated by 185 nucleotides BPHYT_RS23740 and BPHYT_RS23745 are separated by 277 nucleotides BPHYT_RS23745 and BPHYT_RS23750 are separated by 202 nucleotides
BPHYT_RS23735: BPHYT_RS23735 - ABC transporter, at 892,292 to 893,851
_RS23735
BPHYT_RS23740: BPHYT_RS23740 - AraC family transcriptional regulator, at 894,037 to 894,792
_RS23740
BPHYT_RS23745: BPHYT_RS23745 - hypothetical protein, at 895,070 to 895,306
_RS23745
BPHYT_RS23750: BPHYT_RS23750 - phosphonate ABC transporter substrate-binding protein, at 895,509 to 896,597
_RS23750
Position (kb)
894
895 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 893.146 kb on - strand, within BPHYT_RS23735 at 893.722 kb on + strand at 893.829 kb on - strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.934 kb on + strand at 893.935 kb on - strand at 893.969 kb on + strand at 893.970 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 893.973 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.035 kb on - strand at 894.061 kb on + strand at 894.061 kb on + strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.062 kb on - strand at 894.238 kb on - strand, within BPHYT_RS23740 at 894.388 kb on + strand, within BPHYT_RS23740 at 894.389 kb on - strand, within BPHYT_RS23740 at 894.473 kb on + strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.474 kb on - strand, within BPHYT_RS23740 at 894.583 kb on + strand, within BPHYT_RS23740 at 894.835 kb on - strand at 894.939 kb on - strand at 894.981 kb on + strand at 895.040 kb on - strand at 895.040 kb on - strand at 895.040 kb on - strand at 895.061 kb on - strand at 895.305 kb on - strand at 895.635 kb on + strand, within BPHYT_RS23750 at 895.635 kb on + strand, within BPHYT_RS23750 at 895.662 kb on + strand, within BPHYT_RS23750
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 893,146 - BPHYT_RS23735 0.55 -0.1 893,722 + +1.4 893,829 - +0.1 893,934 + -0.7 893,934 + +1.8 893,934 + -0.7 893,934 + +1.8 893,935 - +1.9 893,969 + +0.0 893,970 - -1.3 893,973 - -1.0 893,973 - -1.0 893,973 - -1.1 894,035 - +0.2 894,035 - -0.7 894,035 - +1.0 894,061 + -0.0 894,061 + -1.0 894,062 - -1.0 894,062 - -2.0 894,062 - -3.2 894,238 - BPHYT_RS23740 0.27 -1.4 894,388 + BPHYT_RS23740 0.46 -0.4 894,389 - BPHYT_RS23740 0.47 +0.2 894,473 + BPHYT_RS23740 0.58 -1.7 894,474 - BPHYT_RS23740 0.58 -1.6 894,474 - BPHYT_RS23740 0.58 -3.3 894,474 - BPHYT_RS23740 0.58 -0.4 894,583 + BPHYT_RS23740 0.72 -1.8 894,835 - -0.2 894,939 - +0.7 894,981 + -0.8 895,040 - -0.7 895,040 - -0.6 895,040 - +0.4 895,061 - -1.1 895,305 - -0.2 895,635 + BPHYT_RS23750 0.12 -0.0 895,635 + BPHYT_RS23750 0.12 +0.8 895,662 + BPHYT_RS23750 0.14 -0.1
Or see this region's nucleotide sequence