Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22530

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22525 and BPHYT_RS22530 are separated by 43 nucleotidesBPHYT_RS22530 and BPHYT_RS22535 are separated by 18 nucleotides BPHYT_RS22525: BPHYT_RS22525 - dioxygenase, at 615,060 to 615,899 _RS22525 BPHYT_RS22530: BPHYT_RS22530 - N5,N10-methylene tetrahydromethanopterin reductase, at 615,943 to 617,349 _RS22530 BPHYT_RS22535: BPHYT_RS22535 - methionine ABC transporter ATP-binding protein, at 617,368 to 618,528 _RS22535 Position (kb) 615 616 617 618Strain fitness (log2 ratio) -2 -1 0 1 2at 615.293 kb on + strand, within BPHYT_RS22525at 615.337 kb on - strand, within BPHYT_RS22525at 615.348 kb on - strand, within BPHYT_RS22525at 615.384 kb on + strand, within BPHYT_RS22525at 615.432 kb on + strand, within BPHYT_RS22525at 616.042 kb on + strandat 616.043 kb on - strandat 616.043 kb on - strandat 616.282 kb on + strand, within BPHYT_RS22530at 616.913 kb on - strand, within BPHYT_RS22530at 617.347 kb on + strandat 617.961 kb on + strand, within BPHYT_RS22535

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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615,293 + BPHYT_RS22525 0.28 +1.1
615,337 - BPHYT_RS22525 0.33 +0.4
615,348 - BPHYT_RS22525 0.34 -0.5
615,384 + BPHYT_RS22525 0.39 -0.5
615,432 + BPHYT_RS22525 0.44 -1.8
616,042 + -0.1
616,043 - -0.2
616,043 - +0.4
616,282 + BPHYT_RS22530 0.24 -1.8
616,913 - BPHYT_RS22530 0.69 -1.1
617,347 + -0.1
617,961 + BPHYT_RS22535 0.51 +2.3

Or see this region's nucleotide sequence