Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22215 and BPHYT_RS22220 are separated by 7 nucleotides BPHYT_RS22220 and BPHYT_RS22225 are separated by 158 nucleotides BPHYT_RS22225 and BPHYT_RS22230 are separated by 109 nucleotides
BPHYT_RS22215: BPHYT_RS22215 - hypothetical protein, at 541,743 to 542,828
_RS22215
BPHYT_RS22220: BPHYT_RS22220 - sulfatase, at 542,836 to 544,782
_RS22220
BPHYT_RS22225: BPHYT_RS22225 - hypothetical protein, at 544,941 to 545,489
_RS22225
BPHYT_RS22230: BPHYT_RS22230 - LuxR family transcriptional regulator, at 545,599 to 546,246
_RS22230
Position (kb) 542
543
544
545 Strain fitness (log2 ratio) -2
-1
0
1 at 541.958 kb on - strand at 542.322 kb on + strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.788 kb on + strand at 542.886 kb on + strand at 542.887 kb on - strand at 542.888 kb on + strand at 542.888 kb on + strand at 542.889 kb on - strand at 542.889 kb on - strand at 543.159 kb on - strand, within BPHYT_RS22220 at 543.164 kb on + strand, within BPHYT_RS22220 at 543.591 kb on + strand, within BPHYT_RS22220 at 543.684 kb on - strand, within BPHYT_RS22220 at 543.762 kb on - strand, within BPHYT_RS22220 at 543.795 kb on - strand, within BPHYT_RS22220 at 543.959 kb on + strand, within BPHYT_RS22220 at 543.960 kb on - strand, within BPHYT_RS22220 at 544.062 kb on - strand, within BPHYT_RS22220 at 544.083 kb on - strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.242 kb on + strand, within BPHYT_RS22220 at 544.243 kb on - strand, within BPHYT_RS22220 at 544.312 kb on - strand, within BPHYT_RS22220 at 544.330 kb on - strand, within BPHYT_RS22220 at 544.331 kb on + strand, within BPHYT_RS22220 at 544.332 kb on - strand, within BPHYT_RS22220 at 544.332 kb on - strand, within BPHYT_RS22220 at 544.657 kb on + strand at 544.800 kb on - strand at 544.800 kb on - strand at 544.861 kb on + strand at 544.879 kb on + strand at 544.880 kb on - strand at 545.116 kb on + strand, within BPHYT_RS22225 at 545.117 kb on - strand, within BPHYT_RS22225 at 545.233 kb on + strand, within BPHYT_RS22225 at 545.234 kb on - strand, within BPHYT_RS22225 at 545.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 541,958 - +0.8 542,322 + BPHYT_RS22215 0.53 -0.4 542,420 - BPHYT_RS22215 0.62 -0.7 542,420 - BPHYT_RS22215 0.62 -2.5 542,788 + +0.1 542,886 + -0.1 542,887 - -0.2 542,888 + -0.2 542,888 + -0.6 542,889 - -0.2 542,889 - +0.4 543,159 - BPHYT_RS22220 0.17 +0.3 543,164 + BPHYT_RS22220 0.17 -0.4 543,591 + BPHYT_RS22220 0.39 +0.6 543,684 - BPHYT_RS22220 0.44 +0.8 543,762 - BPHYT_RS22220 0.48 -1.0 543,795 - BPHYT_RS22220 0.49 -2.5 543,959 + BPHYT_RS22220 0.58 -0.1 543,960 - BPHYT_RS22220 0.58 +1.0 544,062 - BPHYT_RS22220 0.63 +1.3 544,083 - BPHYT_RS22220 0.64 +0.1 544,238 + BPHYT_RS22220 0.72 -1.4 544,238 + BPHYT_RS22220 0.72 -0.4 544,238 + BPHYT_RS22220 0.72 -0.6 544,239 - BPHYT_RS22220 0.72 -0.4 544,239 - BPHYT_RS22220 0.72 +0.8 544,239 - BPHYT_RS22220 0.72 +0.6 544,242 + BPHYT_RS22220 0.72 +0.1 544,243 - BPHYT_RS22220 0.72 +0.1 544,312 - BPHYT_RS22220 0.76 -0.3 544,330 - BPHYT_RS22220 0.77 +0.6 544,331 + BPHYT_RS22220 0.77 +0.9 544,332 - BPHYT_RS22220 0.77 +0.6 544,332 - BPHYT_RS22220 0.77 -1.0 544,657 + +0.8 544,800 - +0.5 544,800 - -0.6 544,861 + +0.0 544,879 + -2.5 544,880 - -0.6 545,116 + BPHYT_RS22225 0.32 -0.3 545,117 - BPHYT_RS22225 0.32 +1.5 545,233 + BPHYT_RS22225 0.53 -0.1 545,234 - BPHYT_RS22225 0.53 -0.4 545,435 + +1.4
Or see this region's nucleotide sequence