Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22190 and BPHYT_RS22195 are separated by 22 nucleotides BPHYT_RS22195 and BPHYT_RS22200 are separated by 146 nucleotides BPHYT_RS22200 and BPHYT_RS22205 are separated by 12 nucleotides BPHYT_RS22205 and BPHYT_RS22210 overlap by 7 nucleotides
BPHYT_RS22190: BPHYT_RS22190 - nucleoid-structuring protein H-NS, at 537,221 to 538,072
_RS22190
BPHYT_RS22195: BPHYT_RS22195 - hypothetical protein, at 538,095 to 538,580
_RS22195
BPHYT_RS22200: BPHYT_RS22200 - cardiolipin synthetase, at 538,727 to 539,989
_RS22200
BPHYT_RS22205: BPHYT_RS22205 - hypothetical protein, at 540,002 to 540,958
_RS22205
BPHYT_RS22210: BPHYT_RS22210 - metal ABC transporter substrate-binding protein, at 540,952 to 541,746
_RS22210
Position (kb) 538
539
540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 537.916 kb on - strand, within BPHYT_RS22190 at 538.093 kb on - strand at 538.310 kb on - strand, within BPHYT_RS22195 at 538.414 kb on + strand, within BPHYT_RS22195 at 538.833 kb on - strand at 539.347 kb on + strand, within BPHYT_RS22200 at 539.348 kb on - strand, within BPHYT_RS22200 at 539.412 kb on - strand, within BPHYT_RS22200 at 539.453 kb on - strand, within BPHYT_RS22200 at 539.889 kb on + strand at 540.025 kb on - strand at 540.237 kb on - strand, within BPHYT_RS22205 at 540.237 kb on - strand, within BPHYT_RS22205 at 540.522 kb on + strand, within BPHYT_RS22205 at 540.908 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 537,916 - BPHYT_RS22190 0.82 +0.4 538,093 - -0.5 538,310 - BPHYT_RS22195 0.44 +0.0 538,414 + BPHYT_RS22195 0.66 -0.5 538,833 - -0.0 539,347 + BPHYT_RS22200 0.49 -1.1 539,348 - BPHYT_RS22200 0.49 +0.5 539,412 - BPHYT_RS22200 0.54 -1.4 539,453 - BPHYT_RS22200 0.57 -2.1 539,889 + +3.3 540,025 - -0.0 540,237 - BPHYT_RS22205 0.25 -0.6 540,237 - BPHYT_RS22205 0.25 -2.8 540,522 + BPHYT_RS22205 0.54 -1.5 540,908 - -0.0
Or see this region's nucleotide sequence