Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21995 and BPHYT_RS22000 overlap by 4 nucleotides BPHYT_RS22000 and BPHYT_RS22005 are separated by 23 nucleotides BPHYT_RS22005 and BPHYT_RS22010 are separated by 39 nucleotides
BPHYT_RS21995: BPHYT_RS21995 - (2Fe-2S)-binding protein, at 490,313 to 491,746
_RS21995
BPHYT_RS22000: BPHYT_RS22000 - hypothetical protein, at 491,743 to 492,105
_RS22000
BPHYT_RS22005: BPHYT_RS22005 - ABC transporter permease, at 492,129 to 492,923
_RS22005
BPHYT_RS22010: BPHYT_RS22010 - ABC transporter permease, at 492,963 to 493,877
_RS22010
Position (kb) 491
492
493 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 491.119 kb on - strand, within BPHYT_RS21995 at 491.202 kb on - strand, within BPHYT_RS21995 at 491.232 kb on + strand, within BPHYT_RS21995 at 491.252 kb on + strand, within BPHYT_RS21995 at 491.496 kb on + strand, within BPHYT_RS21995 at 491.788 kb on + strand, within BPHYT_RS22000 at 491.982 kb on + strand, within BPHYT_RS22000 at 492.164 kb on - strand at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.256 kb on + strand, within BPHYT_RS22005 at 492.262 kb on + strand, within BPHYT_RS22005 at 492.263 kb on - strand, within BPHYT_RS22005 at 492.588 kb on + strand, within BPHYT_RS22005 at 493.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 491,119 - BPHYT_RS21995 0.56 +0.4 491,202 - BPHYT_RS21995 0.62 +2.9 491,232 + BPHYT_RS21995 0.64 +0.8 491,252 + BPHYT_RS21995 0.65 -0.9 491,496 + BPHYT_RS21995 0.82 -0.5 491,788 + BPHYT_RS22000 0.12 -2.1 491,982 + BPHYT_RS22000 0.66 -1.0 492,164 - +0.6 492,256 + BPHYT_RS22005 0.16 -0.7 492,256 + BPHYT_RS22005 0.16 -0.7 492,256 + BPHYT_RS22005 0.16 -2.3 492,256 + BPHYT_RS22005 0.16 -0.2 492,262 + BPHYT_RS22005 0.17 +0.4 492,263 - BPHYT_RS22005 0.17 -0.0 492,588 + BPHYT_RS22005 0.58 +0.1 493,006 + +1.7
Or see this region's nucleotide sequence