Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21970 and BPHYT_RS21975 are separated by 219 nucleotides BPHYT_RS21975 and BPHYT_RS21980 are separated by 67 nucleotides BPHYT_RS21980 and BPHYT_RS21985 are separated by 86 nucleotides
BPHYT_RS21970: BPHYT_RS21970 - nucleoside hydrolase, at 485,540 to 486,505
_RS21970
BPHYT_RS21975: BPHYT_RS21975 - 2-dehydropantoate 2-reductase, at 486,725 to 487,660
_RS21975
BPHYT_RS21980: BPHYT_RS21980 - hypothetical protein, at 487,728 to 488,027
_RS21980
BPHYT_RS21985: BPHYT_RS21985 - spermidine/putrescine ABC transporter substrate-binding protein, at 488,114 to 489,160
_RS21985
Position (kb) 486
487
488 Strain fitness (log2 ratio) -2
-1
0
1
2 at 485.822 kb on - strand, within BPHYT_RS21970 at 486.266 kb on + strand, within BPHYT_RS21970 at 486.267 kb on - strand, within BPHYT_RS21970 at 486.342 kb on + strand, within BPHYT_RS21970 at 486.636 kb on + strand at 486.736 kb on - strand at 486.738 kb on + strand at 487.046 kb on - strand, within BPHYT_RS21975 at 487.132 kb on + strand, within BPHYT_RS21975 at 487.631 kb on + strand at 487.632 kb on - strand at 487.632 kb on - strand at 488.050 kb on - strand at 488.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 485,822 - BPHYT_RS21970 0.29 +0.0 486,266 + BPHYT_RS21970 0.75 -1.3 486,267 - BPHYT_RS21970 0.75 -0.5 486,342 + BPHYT_RS21970 0.83 +1.0 486,636 + +0.0 486,736 - +0.9 486,738 + +0.2 487,046 - BPHYT_RS21975 0.34 -1.8 487,132 + BPHYT_RS21975 0.43 -1.1 487,631 + +0.9 487,632 - -0.2 487,632 - +0.0 488,050 - +2.0 488,173 + +1.0
Or see this region's nucleotide sequence