Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21850 and BPHYT_RS21855 are separated by 98 nucleotides BPHYT_RS21855 and BPHYT_RS21860 are separated by 778 nucleotides
BPHYT_RS21850: BPHYT_RS21850 - pyrimidine reductase, at 456,173 to 456,850
_RS21850
BPHYT_RS21855: BPHYT_RS21855 - hypothetical protein, at 456,949 to 457,716
_RS21855
BPHYT_RS21860: BPHYT_RS21860 - hypothetical protein, at 458,495 to 458,953
_RS21860
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1
2 at 456.182 kb on + strand at 456.183 kb on - strand at 456.184 kb on + strand at 456.184 kb on + strand at 456.184 kb on + strand at 456.185 kb on - strand at 456.185 kb on - strand at 456.264 kb on - strand, within BPHYT_RS21850 at 456.264 kb on - strand, within BPHYT_RS21850 at 456.416 kb on + strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 456.528 kb on - strand, within BPHYT_RS21850 at 457.227 kb on - strand, within BPHYT_RS21855 at 457.285 kb on - strand, within BPHYT_RS21855 at 457.285 kb on - strand, within BPHYT_RS21855 at 457.559 kb on + strand, within BPHYT_RS21855 at 457.560 kb on - strand, within BPHYT_RS21855 at 457.560 kb on - strand at 457.560 kb on - strand, within BPHYT_RS21855 at 457.787 kb on + strand at 457.788 kb on - strand at 457.879 kb on - strand at 457.914 kb on + strand at 458.069 kb on + strand at 458.074 kb on - strand at 458.199 kb on - strand at 458.203 kb on + strand at 458.203 kb on + strand at 458.204 kb on - strand at 458.323 kb on + strand at 458.324 kb on - strand at 458.444 kb on + strand at 458.445 kb on - strand at 458.497 kb on - strand at 458.653 kb on - strand, within BPHYT_RS21860
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 456,182 + -2.2 456,183 - -0.3 456,184 + +1.8 456,184 + +1.2 456,184 + -0.9 456,185 - +1.4 456,185 - -0.8 456,264 - BPHYT_RS21850 0.13 +0.6 456,264 - BPHYT_RS21850 0.13 -0.2 456,416 + BPHYT_RS21850 0.36 -0.1 456,528 - BPHYT_RS21850 0.52 +0.6 456,528 - BPHYT_RS21850 0.52 -0.6 456,528 - BPHYT_RS21850 0.52 -1.9 457,227 - BPHYT_RS21855 0.36 -1.2 457,285 - BPHYT_RS21855 0.44 -1.4 457,285 - BPHYT_RS21855 0.44 -2.2 457,559 + BPHYT_RS21855 0.79 -2.0 457,560 - BPHYT_RS21855 0.80 +0.6 457,560 - +0.0 457,560 - BPHYT_RS21855 0.80 -1.0 457,787 + -0.8 457,788 - -1.1 457,879 - -0.4 457,914 + -0.7 458,069 + +0.1 458,074 - +1.5 458,199 - -0.8 458,203 + -0.4 458,203 + +1.4 458,204 - -0.2 458,323 + +0.6 458,324 - +0.1 458,444 + -1.2 458,445 - -1.2 458,497 - -0.4 458,653 - BPHYT_RS21860 0.34 +0.2
Or see this region's nucleotide sequence