Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21350 and BPHYT_RS21355 are separated by 225 nucleotides BPHYT_RS21355 and BPHYT_RS21360 are separated by 221 nucleotides
BPHYT_RS21350: BPHYT_RS21350 - ADP-glucose pyrophosphorylase, at 348,294 to 349,058
_RS21350
BPHYT_RS21355: BPHYT_RS21355 - major facilitator transporter, at 349,284 to 350,687
_RS21355
BPHYT_RS21360: BPHYT_RS21360 - gamma-glutamyltranspeptidase, at 350,909 to 352,642
_RS21360
Position (kb) 349
350
351 Strain fitness (log2 ratio) -2
-1
0
1
2 at 348.865 kb on - strand, within BPHYT_RS21350 at 348.929 kb on + strand, within BPHYT_RS21350 at 349.238 kb on + strand at 349.440 kb on + strand, within BPHYT_RS21355 at 349.440 kb on + strand, within BPHYT_RS21355 at 349.441 kb on - strand, within BPHYT_RS21355 at 349.441 kb on - strand, within BPHYT_RS21355 at 349.441 kb on - strand, within BPHYT_RS21355 at 349.441 kb on - strand, within BPHYT_RS21355 at 349.693 kb on - strand, within BPHYT_RS21355 at 349.714 kb on + strand, within BPHYT_RS21355 at 349.715 kb on - strand, within BPHYT_RS21355 at 349.758 kb on + strand, within BPHYT_RS21355 at 349.758 kb on + strand, within BPHYT_RS21355 at 349.758 kb on + strand, within BPHYT_RS21355 at 349.758 kb on + strand, within BPHYT_RS21355 at 349.759 kb on - strand, within BPHYT_RS21355 at 349.759 kb on - strand, within BPHYT_RS21355 at 349.759 kb on - strand, within BPHYT_RS21355 at 349.759 kb on - strand, within BPHYT_RS21355 at 349.759 kb on - strand at 349.759 kb on - strand, within BPHYT_RS21355 at 349.988 kb on - strand, within BPHYT_RS21355 at 350.013 kb on + strand, within BPHYT_RS21355 at 350.189 kb on + strand, within BPHYT_RS21355 at 350.190 kb on - strand, within BPHYT_RS21355 at 350.313 kb on + strand, within BPHYT_RS21355 at 350.685 kb on + strand at 350.813 kb on + strand at 350.818 kb on + strand at 350.818 kb on + strand at 350.819 kb on - strand at 350.819 kb on - strand at 350.819 kb on - strand at 350.819 kb on - strand at 350.820 kb on + strand at 350.820 kb on + strand at 350.821 kb on - strand at 350.821 kb on - strand at 350.853 kb on + strand at 350.854 kb on - strand at 350.873 kb on + strand at 350.873 kb on + strand at 350.929 kb on + strand at 351.209 kb on + strand, within BPHYT_RS21360 at 351.209 kb on + strand, within BPHYT_RS21360 at 351.209 kb on + strand, within BPHYT_RS21360 at 351.210 kb on - strand, within BPHYT_RS21360 at 351.210 kb on - strand, within BPHYT_RS21360 at 351.210 kb on - strand, within BPHYT_RS21360 at 351.278 kb on + strand, within BPHYT_RS21360 at 351.278 kb on + strand, within BPHYT_RS21360 at 351.278 kb on + strand, within BPHYT_RS21360 at 351.278 kb on + strand, within BPHYT_RS21360 at 351.279 kb on - strand, within BPHYT_RS21360 at 351.394 kb on + strand, within BPHYT_RS21360 at 351.395 kb on - strand, within BPHYT_RS21360 at 351.518 kb on - strand, within BPHYT_RS21360 at 351.521 kb on + strand, within BPHYT_RS21360 at 351.521 kb on + strand, within BPHYT_RS21360 at 351.615 kb on - strand, within BPHYT_RS21360
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 348,865 - BPHYT_RS21350 0.75 -0.2 348,929 + BPHYT_RS21350 0.83 -2.6 349,238 + -0.6 349,440 + BPHYT_RS21355 0.11 +0.3 349,440 + BPHYT_RS21355 0.11 +0.8 349,441 - BPHYT_RS21355 0.11 +1.3 349,441 - BPHYT_RS21355 0.11 -0.7 349,441 - BPHYT_RS21355 0.11 -1.3 349,441 - BPHYT_RS21355 0.11 +0.5 349,693 - BPHYT_RS21355 0.29 -0.9 349,714 + BPHYT_RS21355 0.31 -0.3 349,715 - BPHYT_RS21355 0.31 -1.9 349,758 + BPHYT_RS21355 0.34 +0.4 349,758 + BPHYT_RS21355 0.34 -1.5 349,758 + BPHYT_RS21355 0.34 -0.8 349,758 + BPHYT_RS21355 0.34 +0.0 349,759 - BPHYT_RS21355 0.34 -0.5 349,759 - BPHYT_RS21355 0.34 -0.5 349,759 - BPHYT_RS21355 0.34 -1.1 349,759 - BPHYT_RS21355 0.34 +1.2 349,759 - -0.5 349,759 - BPHYT_RS21355 0.34 +0.2 349,988 - BPHYT_RS21355 0.50 +0.3 350,013 + BPHYT_RS21355 0.52 -0.1 350,189 + BPHYT_RS21355 0.64 -1.5 350,190 - BPHYT_RS21355 0.65 -0.5 350,313 + BPHYT_RS21355 0.73 +1.2 350,685 + -1.1 350,813 + -0.0 350,818 + +2.7 350,818 + +0.3 350,819 - -0.2 350,819 - -1.8 350,819 - -1.1 350,819 - +0.7 350,820 + +0.9 350,820 + -0.4 350,821 - -1.0 350,821 - +0.7 350,853 + -0.9 350,854 - +0.1 350,873 + -0.5 350,873 + -0.5 350,929 + +1.9 351,209 + BPHYT_RS21360 0.17 +0.6 351,209 + BPHYT_RS21360 0.17 -0.0 351,209 + BPHYT_RS21360 0.17 -0.7 351,210 - BPHYT_RS21360 0.17 -0.2 351,210 - BPHYT_RS21360 0.17 -0.2 351,210 - BPHYT_RS21360 0.17 -0.7 351,278 + BPHYT_RS21360 0.21 +0.3 351,278 + BPHYT_RS21360 0.21 -0.0 351,278 + BPHYT_RS21360 0.21 -0.7 351,278 + BPHYT_RS21360 0.21 +0.2 351,279 - BPHYT_RS21360 0.21 -0.2 351,394 + BPHYT_RS21360 0.28 -1.0 351,395 - BPHYT_RS21360 0.28 +0.8 351,518 - BPHYT_RS21360 0.35 +0.8 351,521 + BPHYT_RS21360 0.35 +0.9 351,521 + BPHYT_RS21360 0.35 -1.1 351,615 - BPHYT_RS21360 0.41 -0.4
Or see this region's nucleotide sequence