Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20605 and BPHYT_RS20610 are separated by 39 nucleotides BPHYT_RS20610 and BPHYT_RS20615 are separated by 165 nucleotides
BPHYT_RS20605: BPHYT_RS20605 - LysR family transcriptional regulator, at 181,701 to 182,837
_RS20605
BPHYT_RS20610: BPHYT_RS20610 - LysR family transcriptional regulator, at 182,877 to 183,788
_RS20610
BPHYT_RS20615: BPHYT_RS20615 - 2-nitropropane dioxygenase, at 183,954 to 185,054
_RS20615
Position (kb) 182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 182.204 kb on - strand, within BPHYT_RS20605 at 182.227 kb on - strand, within BPHYT_RS20605 at 182.496 kb on + strand, within BPHYT_RS20605 at 182.496 kb on + strand, within BPHYT_RS20605 at 182.497 kb on - strand, within BPHYT_RS20605 at 182.652 kb on + strand, within BPHYT_RS20605 at 182.878 kb on + strand at 182.879 kb on - strand at 183.043 kb on + strand, within BPHYT_RS20610 at 183.112 kb on + strand, within BPHYT_RS20610 at 183.113 kb on - strand, within BPHYT_RS20610 at 183.197 kb on - strand, within BPHYT_RS20610 at 183.466 kb on + strand, within BPHYT_RS20610 at 183.467 kb on - strand, within BPHYT_RS20610 at 183.578 kb on - strand, within BPHYT_RS20610 at 183.745 kb on - strand at 183.924 kb on + strand at 183.924 kb on + strand at 183.924 kb on + strand at 183.924 kb on + strand at 183.925 kb on - strand at 183.925 kb on - strand at 183.929 kb on + strand at 183.930 kb on - strand at 184.257 kb on + strand, within BPHYT_RS20615 at 184.294 kb on - strand, within BPHYT_RS20615 at 184.294 kb on - strand, within BPHYT_RS20615 at 184.499 kb on + strand, within BPHYT_RS20615 at 184.499 kb on + strand, within BPHYT_RS20615
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 182,204 - BPHYT_RS20605 0.44 -0.7 182,227 - BPHYT_RS20605 0.46 -0.7 182,496 + BPHYT_RS20605 0.70 +0.6 182,496 + BPHYT_RS20605 0.70 +0.8 182,497 - BPHYT_RS20605 0.70 +0.1 182,652 + BPHYT_RS20605 0.84 -1.3 182,878 + +1.6 182,879 - -0.1 183,043 + BPHYT_RS20610 0.18 +0.2 183,112 + BPHYT_RS20610 0.26 -1.9 183,113 - BPHYT_RS20610 0.26 +0.0 183,197 - BPHYT_RS20610 0.35 +0.3 183,466 + BPHYT_RS20610 0.65 +2.4 183,467 - BPHYT_RS20610 0.65 +0.1 183,578 - BPHYT_RS20610 0.77 -0.7 183,745 - -0.1 183,924 + -2.3 183,924 + +0.1 183,924 + -0.4 183,924 + +0.1 183,925 - -1.0 183,925 - -1.8 183,929 + -0.6 183,930 - -0.2 184,257 + BPHYT_RS20615 0.28 -1.4 184,294 - BPHYT_RS20615 0.31 -0.0 184,294 - BPHYT_RS20615 0.31 +0.2 184,499 + BPHYT_RS20615 0.50 -3.2 184,499 + BPHYT_RS20615 0.50 +0.6
Or see this region's nucleotide sequence