Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20225 and BPHYT_RS20230 overlap by 4 nucleotides BPHYT_RS20230 and BPHYT_RS20235 overlap by 4 nucleotides BPHYT_RS20235 and BPHYT_RS20240 overlap by 4 nucleotides BPHYT_RS20240 and BPHYT_RS20245 are separated by 5 nucleotides
BPHYT_RS20225: BPHYT_RS20225 - taurine catabolism dioxygenase tauD/tfdA, at 81,933 to 82,967
_RS20225
BPHYT_RS20230: BPHYT_RS20230 - ABC transporter, at 82,964 to 83,830
_RS20230
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
BPHYT_RS20240: BPHYT_RS20240 - iron reductase, at 85,932 to 86,750
_RS20240
BPHYT_RS20245: BPHYT_RS20245 - iron ABC transporter substrate-binding protein, at 86,756 to 87,856
_RS20245
Position (kb) 83
84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 82.914 kb on + strand at 82.915 kb on - strand at 83.809 kb on - strand at 83.884 kb on + strand at 84.160 kb on - strand, within BPHYT_RS20235 at 84.427 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.481 kb on - strand, within BPHYT_RS20235 at 86.202 kb on + strand, within BPHYT_RS20240 at 86.203 kb on - strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.206 kb on - strand, within BPHYT_RS20240 at 86.478 kb on + strand, within BPHYT_RS20240 at 86.650 kb on + strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.655 kb on + strand, within BPHYT_RS20240 at 86.702 kb on - strand at 86.867 kb on + strand, within BPHYT_RS20245
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 82,914 + -1.8 82,915 - -1.3 83,809 - -0.3 83,884 + -1.6 84,160 - BPHYT_RS20235 0.16 +0.3 84,427 + BPHYT_RS20235 0.28 -1.8 85,480 + BPHYT_RS20235 0.78 -2.5 85,480 + BPHYT_RS20235 0.78 -1.4 85,480 + BPHYT_RS20235 0.78 -0.3 85,481 - BPHYT_RS20235 0.78 -1.8 86,202 + BPHYT_RS20240 0.33 -1.3 86,203 - BPHYT_RS20240 0.33 -2.8 86,205 + BPHYT_RS20240 0.33 +0.0 86,205 + BPHYT_RS20240 0.33 -2.2 86,206 - BPHYT_RS20240 0.33 -1.7 86,478 + BPHYT_RS20240 0.67 +1.3 86,650 + BPHYT_RS20240 0.88 +0.8 86,651 - BPHYT_RS20240 0.88 -2.0 86,651 - BPHYT_RS20240 0.88 +0.7 86,651 - BPHYT_RS20240 0.88 -0.0 86,655 + BPHYT_RS20240 0.88 +0.1 86,702 - -2.7 86,867 + BPHYT_RS20245 0.10 +1.3
Or see this region's nucleotide sequence